APP (Y188)
Epitomics
monoclonal
Mouse
(liquid, Tris-Glycine, NaCl, Glycerol, BSA, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresponding to residues in the NPXY motif of human APP
specifically recognizes APP and its isoforms; will not recognize either of the two Amyloid Beta Protein (Aβ4) variants, Aβ1 -40 or Aβ1 -42
-
APP, N-terminal
Calbiochem EMD Biosciences
polyclonal
IgG
Goat
(liquid, neat serum, no preservative)
-
Human
immunogen = synthetic peptide (Y E V H H Q K L V F F A E D V) corresp. to aa 44-63 of human APP
APP (~110-135 kDa)
-
APP (DE2B4)
Abcam
monoclonal
IgG1
Mouse
(liquid, supernatant, azide)
-
Cow, Pig, Human, Monkey
immunogen = synthetic peptide corresp. to aa 1-17 of the mature human Amyloid Precursor Protein, conjugated to KLH.
reacts with β-amyloid peptide and secreted APP (alpha-secretase cleaved); has very weak recognition of full length APP and we do not recommend it for this purpose; does however strongly recognize alpha-secretase cleaved soluble APP as well as b-amyloid peptide (40 and 42)
-
β-APP
Zymed/ Invitrogen
polyclonal
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse
immunogen = synthetic peptide derived from the C-terminal region of the human and mouse amyloid-β precursor protein (APP695)
APP full length (~100kDa) and C99 C-terminal cleavage frag (~10-12kDa); cross-react with APLP2
-
β-APP
Zymed/ Invitrogen
polyclonal
Rabbit
(affinity purified, PBS, azide)
-
Pig, Human, Mouse, Rat
Immunogen = synthetic peptide of 22 aa from the C-terminus of human β-APP
All three forms of full length APP and N-terminal truncated forms of β-APP. Detects C-terminal membrane fragment of β-APP that remains after α -or β-secretase cleavage
-
APP A4 (22C11)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
-
Fish (various), Dog, Pig, Human, Monkey, Mouse, Rat
immunogen = purified rhN-terminal epitope (aa 66-81) on the pre-A4 molecule, fusion protein
human pre-A4 APP; recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa; cross reacts with APLP2
-
Soluble Amyloid Oligomers
A11(paper comparing A11 and OC antibodies;source paper for A11; Charles G Glabe, University of California, Irvine)References:Lefterov, 2010
polyclonal
Rabbit
(serum)
-
Human
immunogen = soluble Amyloid oligomers
generic epitope common to prefibrillar oligomers and not fibrils, monomers or natively folded precursor proteins. Stains small focal or punctuate deposits in AD tissue does not stain diffuse plaques or other plaque types. Stains Aβ and polyQ prefibrillar oligomers.
-
Amyloid fibrils and fibrillar oligomers
OC(paper on antibodies;paper on oligimerization; Charles G Glabe, University of California, Irvine)References:Lefterov, 2010Clinton, 2010Blurton-Jones, 2009
polyclonal
Rabbit
(serum)
-
Human
immunogern = a morphologically homogeneous population of Aβ42 fibrils
specifically recognizes fibrils, but not random coil monomer or prefibrillar oligomers; also recognizes 100,000 × G soluble fibrillar oligomers ranging in size from dimer to greater than 250 kDa on western blots. The fibrillar oligomers recognized by OC are immunologically distinct from prefibrillar oligomers recognized by A11, even though their sizes overlap broadly
-
Aβ 1-16
IC16(Claus U. Pietrzik,Institute of Physiological Chemistry and Pathobiochemistry, MolecularNeurodegeneration, Johannes-Gutenberg-University Mainz, Mainz, Germany)paper)Reference: Richter L, 2010
monoclonal
Mouse
-
Human
immunogen = synthetic human Ab peptide 1–16, cross-linked to keyhole limpet hemocyanin
recognizes residues 1-16 of Amyloid β. Hybridoma cells were generated by standard procedures and screened for recognition of HSA cross-linked to Ab 1-16by ELISA; recognition of synthetic Ab 1–42 ona standardized dot blot procedure was confirmed and the epitope mapped to Ab 1–8 using a peptide array spanning the entire sequence of Ab
-
Aβ 40, C-terminus
BAP-17(Manfred Brockhaus, F. Hoffmann-La Roche Ltd., Basel,Paper)
monoclonal
IgG1
Mouse
(purified from spent hybridoma medium and conjugated to horseradish peroxidase)
-
Human
immunogen = the peptide CMVGGVV conjugatedto keyhole limpet hemocyanin
specific for Aβ40; no cross-reactivity observed with Ab39, Ab42 or Ab43. Ab41 showed a signal accounting for about10% of the cognate Ab40 signal
-
Aβ 42, C-terminus
BAP-15(Manfred Brockhaus, F. Hoffmann-La Roche Ltd., Basel,Paper)Reference: Richter L, 2010
monoclonal
Mouse
(purified from spent hybridoma medium and conjugated to horseradish peroxidase)
-
Human
immunogen = the peptide CGGVVIA conjugatedto keyhole limpet hemocyanin
specific for Aβ42; no cross-reactivity observed with Ab39, Ab40 or Ab43. Ab41 showed a signal accounting for about10% of the cognate Ab42 signal
-
Aβ (BAN50)
BAN-50(N Suzuki,Takada Chemical Industries)References:Wang S, 2010Fukumoto, 2010Suzuki, 1994
monoclonal
Mouse
-
Human, Mouse
raised against Aβ 1-16 (epitope at aa 1-10)
amyloid-beta
-
Aβ 40 (G2-10)
Genetics Company, Inc.
monoclonal
IgG2b ,κ
Mouse
(liquid, supernatant, stabilizing solution, affinity purified, conj. HRP)
-
Dog, Human, Guinea Pig, Mouse, Rat, Cynomolgus
C-terminal epitope mapping corr. to amino acid residues 31-40 of human Aβ peptide
recognizes Aβ-peptide, aa 31-40; selective for C-terminal of Aβ 1-40; not Aβ 1-38, Aβ 1-39, Aβ 1-42, Aβ 1-43 or Aβ 1-44
-
Aβ 42 (G2-13)
Genetics Company, Inc.
monoclonal
IgG1 ,κ
Mouse
(liquid, stabilizing solution, no azide, affinity purified, conj. HRP)
-
Dog, Human, Guinea Pig, Mouse, Rat, Cynomolgus
immunogen = Aβ 1-42 peptide at the C-terminus
C-terminal of Aβ 1-42 peptide; not Aβ 1-38, Aβ 1-39, Aβ 1-40; low cross reactivity to Aβ 43 (10%)and Aβ 44 (13%)
-
Aβ[4-10] and APPβ (W0-2)
Genetics Company, Inc.
monoclonal
IgG2a ,κ
Mouse
(supernatant IgG, Tris, azide, affinity purifiedAlso available Biotin or peroxidase conjugated)
-
Human
N-terminal: Epitope mapping corr. to amino acid residues 4-10 of human Aβ-peptide
High affinity to the N-terminal of human Amyloid β peptides Aβ 1-38, Aβ 1-39, Aβ 1-40, Aβ 1-43 and Aβ 1-44
-
COX-IV
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A purified, HEPES, NaCl, BSA, glycerol)
-
Bovine, Human, Monkey, Mouse, Rat
immunogen = synthetic peptide corresponding to residues surrounding Lys29 of human COX IV
detects endogenous levels of total COX IV protein
-
COX-IV (3E11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, protein-A purified, HEPES, NaCl, BSA, glycerol, azide.Also available Biotinylated, cat# 6014HRP conj., cat# 5247Alexa Fluor® 488 conj., cat# 4853)
-
Bovine, Zebra fish, Pig, Human, Monkey, Rat
immunogen = synthetic peptide corresponding to residues surrounding Lys29 of human COX IV
detects endogenous levels of total COX IV protein
-
Cofilin phos Ser3
Abcam
polyclonal
IgG
Rabbit
(liquid, affinity purified, BSA, PBS, azide)
-
Dog, Human, Rat
immunogen = synthetic peptide derived from a region of human cofilin that contains serine 3; sequence is conserved in mouse and rat
detects cofilin protein phosphorylized at Serine 3
-
Myosin light chain, phos ser19 (MF-20)
Cell Signaling
monoclonal
IgG1
Mouse
(mono: liquid, HEPES, BSA, glycerol, azide;poly: liquid, protein-A affinity purified, HEPES, saline, BSA, glycerol, azide;)
-
Bovine, Pig, Human, Mouse, Rat
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2
detect endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. These antibodies do not cross-react with the cardiac isoform of myosin light chain 2
-
GSK-3β pSer9 (5B3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
immunogen = a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β
recognizes endogenous levels of GSK-3 only when phosphorylated at Ser9 of GSK-3β may cross weakly with the phosphorylated form of GSK-3α due to high sequence homology
-
GSK-3β (7/GSK-3b)
BD Transduction
monoclonal
IgG1
Mouse
(aqueous buffered solution, glycerol, BSA, azide)
-
Dog, Chicken, Human, Mouse, Rat
Immunogen = rat GSK-3β, aa 1-160
detects GSK3β, 46 kDa
-
HSPA1A /HSP70-1
Pierce Thermo Scientific
unknown
Unknown Host
-
Human
-
specific for HSP70-1
-
HSP-70 (C92F3A-5)
A B R - Thermo Scientific
monoclonal
Mouse
(liquid, protein-G affinity purified, PBS, azide)
-
Bovine, Dog, Ovine, Porcine, Rabbit, Chicken, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, C. elegans, Drosophila
immunogen = human Hsp70 purified from HeLa cells, corresponding to amino acids 436-503
specific for HSP-70; does not cross-react with Hsc70
-
HSP-70 (2A4)
A B R - Thermo Scientific
monoclonal
IgM
Mouse
(liquid, purified, PBS, azide)
-
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila
immunogen = human recombinant HSP70 over expressed in E. coli
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72
-
HSP-70 (5A5)
A B R - Thermo Scientific
monoclonal
IgG1
Mouse
(liquid, purified, PBS, azide)
-
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila
immunogen = human recombinant HSP70 over expressed in E. coli
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72
-
HSP-70 (JG-1)
A B R - Thermo Scientific
monoclonal
IgG3
Mouse
(liquid, protein-G affinity purified, PBS, azide)
-
Dog, Primate (various), Human, Mouse
immunogen = Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70
detects mitochondrial heat shock protein 70 kDa (mtHSP70)
-
Hsp60 (24/HSP60)
BD Transduction
monoclonal
IgG1
Mouse
(aqueous, buffered solution, BSA, azide, glycerol)
-
Human, Mouse, Rat
immunogen = human Hsp60 aa. 1-200
detects Hsp60
-
ERK1/2 p44/42 MAPK (L34F12)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide derived from the sequences of rat p44/42 MAP kinase
Recognizes endogenous levels of total p44/42 MAP kinase (ERK1/2) protein. Does not cross-react with JNK/SAPK or p38 MAP kinase.
-
ERK1/2 p44/42 phos Thr202/Tyr204 (E10)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, BSA, HEPES, NaCl, glycerol, azide.See also Cat# 4374, Alexa Fluor® 488 Conjugate;Cat# 4375, Alexa Fluor® 647 Conjugate;)
-
Bovine, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, Drosophila
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Detects endogenous levels pf p44/42 MAP kinase (ERK1/2) dually phosphorylated at Thr202 and tyr204. Does not cross-react with the corresp. phospho-residues of SAPK/JNK or p38 MAP kinase
-
Aggrecan (HAG7D4)
Acris Antibodies
monoclonal
IgG1
Rat
(liquid, supernatant, Tris/HCl, foetal calf serum, azide)
-
Bovine, Human
immunogen = purified Human Articular Cartilage Aggrecan
recognizes epitopes within the N-terminal G1-IGD-G2 region of Aggrecan, that has been identified as a cluster 1 isoform
-
Drp-1 /Dynamin related protein 1
Pierce Thermo Scientific
polyclonal
Rabbit
(liquid, affinity purified, Tris-glycine, saline, azide)
-
Primate (various), Human, Hamster, Mouse
Immunogen = synthetic peptide corresponding to a region within residues 1-100 of human DRP1 (16986) or residues 500-600 of human DRP1 (16987)
detects Dynamin related protein 1
-
BAF170 (E-6)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse
immunogen = amino acids 1093-1208 near the C-terminus of BAF170 of human origin
detects BAF170
-
Neurofilament 150&200kD (JJ29?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (D21?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain
150 & 200 kD, phosphatase sensitive
-
Neurofilament 150&200kD (JJ28?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (JJ32?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (JJ42?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific
-
Neurofilament 150&200kD (JJ47?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya
-
Neurofilament 150&200kD (JJ51?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya
-
Neurofilament 150&200kD (JJ8?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150kD (C21?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain Neurofilament
NF 150 kD and GFAP, amino terminal rod, phosphatase insensitive,does not stain astrocytes
-
Neurofilament 200kD (A9?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain
NF 200 kD, axon specific, phosphatase sensitive
-
Neurofilament 200kD (FF1?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, COOH terminal tail domain, phosphatase sensitive
-
Neurofilament 200kD (II19?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, cell bodies positive, phosphatase insensitive
-
Neurofilament 200kD (II20?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, cell bodies positive, phosphatase insensitive
-
Neurofilament 200kD (II20?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, phosphatase sensitive
-
Neurofilament H
RMO24(Virginia Lee, U of Penn, CNDR, Philadelphia, PA)used in paper by Giasson, 2002
monoclonal
-
-
phosphorylated NF heavy subunit
-
Tom20 (FL-145)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide corresp. to amino acids 1-145 representing full length Tom20 of human origin
recognizes Tom20
-
Opa1 (V-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse
immunogen = apeptide mapping within an internal region of OPA1 (dynamin family) of human origin.
for detection of Optic atrophy 1 gene protein
-
Mitofilin
Chemicon and Upstate are Millipore
polyclonal
IgG1
Rabbit
(liquid, raw rabbit serum)
-
Human, Mouse, Rat
-
recognizes mitofilin, mitochondrion inner membrane protein
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, FITC conj.)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
Omi/HtrA2 (229926)
R&D Systems
monoclonal
IgG2a
Mouse
(filtered solution, protein-G affinity purified, PBS, trehalose, lyophilized)
-
Human
immunogen = purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhHtrA2; aa 134-458)
detects the mitochondria-processed form of endogenous human HtrA2/Omi.
-
Tim23 (32/Tim23)
BD Transduction
monoclonal
IgG2a
Mouse
(Aqueous buffered solution, affinity purified, BSA, glycrol, azide)
-
Human, Mouse, Rat
immunogen = rat Tim23 aa. 5-126.
detects Tim23, 23 kDa by WB
-
Lamp-2
Sigma Aldrich
polyclonal
Rabbit
(buffered aqueous solution, affinity isolated, PBS, azide)
-
Mouse, Rat
immunogen = a synthetic peptide corresponding to amino acid residues 404-415 of mouse LAMP2 with N-terminal added cysteine, conjugated to KLH
detects LAMP-2
-
Omi/HtrA2
R&D Systems
polyclonal
IgG
Rabbit
(filtered solution, affinity purified, PBS, trehalose, lyophilized)
-
Human, Mouse
immunogen = E. coliderived recombinant human HTRA2/Omi Ala134Glu45
detects full length and mitochondria processed HTRA2/Omi
-
SIRT1 (2G1/F7)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, glycrol, azide, Protein G purified)
-
Human
Immunogen = peptide corresp. to aa 551-599 of human Sirt1
SIRT1(120kDa by WB). An additional unidentified band was detected (100 kDa).
-
Sir2/SIRT1 (10E4)
Chemicon and Upstate are Millipore
monoclonal
IgG
Rabbit
(liquid, Tris-Glycine, saline, BSA, glycrol, azide)
-
Human
Immunogen = synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2
recognizes SIRT1(~110 kDa by WB); does not cross-react with other sirtuin family members.
-
SIRT1 (10E4)
Chemicon and Upstate are Millipore
monoclonal
IgG,κ
Mouse
(liquid, purified, Tris-Glycine, saline, azide)
-
Human, Mouse
Immunogen = His-tagged recombinant protein corresponding to human SIRT1; mouse sequence is 100% identical to immunogen
recognizes SIRT1
-
SIRT1/Sir2
Chemicon and Upstate are Millipore
polyclonal
IgG
Rabbit
(liquid, purified, Tris-Glycine, saline, azide, glycerol)
-
Human, Mouse
Immunogen = His-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α
recognizes SIRT1, ~110 kDa
-
acetylated Tau /ac-tau
Ab708Li Gan, Gladstone Institute of Neurological DiseaseAssistant Professor of NeurologyUniversity of California, San FranciscoReference: Min SW, 2010
polyclonal
IgG2b
Rabbit
-
Human, Rat
immunogen = a synthetic tau peptide (amino acids 160–182 for 2N4R tau isoform) containing acetylated lysines at positions 163 and 174 and 180
Ab708 specifically recognizes tau acetylated by p300 under cell-free conditions. In HEK293T cells transfected with tau, overexpression of p300 markedly elevatedthe levels of ac-tau detected with Ab708 while the increase in the levels of t-tau was modest, suggesting that Ab708 preferentially recognizes p300-induced ac-tau in cultured cells. Ab708 recognizes human tau acetylated at positions 163, 174, or 180 and possibly other acetylated lysines on tau, but not nonacetylated tau
-
acetylated Tau /ac-tau
9ABLi Gan, Gladstone Institute of Neurological DiseaseAssistant Professor of NeurologyUniversity of California, San FranciscoReference: Min SW, 2010
polyclonal
Rabbit
-
Human, Mouse
immunogen = the microtubule-binding region aa 264–287 (ENLKHQPGGG VQIINKLDLSNV), which is 100% conserved between mouse and human
9AB recognizes recombinant tau acetylated by GST-p300, but not tau incubated with GST alone, suggesting that 9AB does not cross-react with non-ac-tau. 9AB also preferentially recognizes p300-induced ac-tau in cultured cells. In mouse brains, 9AB detected low levels of ac-tau. Does not recognize nonacetylated recombinant tau
-
Tau (12E8)
Philip Dolan, Prothena Biosciences, South San Francisco, CA
monoclonal
Mouse
-
Mammal (various), Human, Zebra fish, Drosophila, C. elegans
immunogen = tau phosphorylated at S262 in KXGS motif of the 4th repeat and/or Ser356
tau phosphorylated at S262 and/or S356
-
Tau (TAU-5)
Abcam
monoclonal
IgG1
Mouse
(liquid, IgG fraction, PBS, azide.For BSA and azide free, see ab80579)
-
Human
immunogen = purified bovine microtubule associated proteins
specific to 45-68 kDa proteins identified as tau proteins; no cross with tubulin or other MAPs; phospho. as well as non-phospho. forms of tau
-
Tau pT205
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 205
tau phosphorylated at threonine 205
-
Tau pS422
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 422
tau phosphorylated at serine 422
-
Tau (AT270)
Pierce Thermo Scientific
monoclonal
IgG1 κ
Mouse
(supernatant, affinity purified, no carrier or preservativeAlso available biotin conj., MN-1050B)
-
Human
immunogen == partially purified human PHF tau, aa. around phosphorylated Thr181
PHF tau, tamgles and neurofilaments; no cross with recomb. unphospho tau; weak cross with normal tau
-
Tau, C-term 4 repeats
DAKO
polyclonal
Ig
Rabbit
(serum, purified Ig fraction, azide)
-
Human, Mouse, Rat
immunogen = C-terminal part of human tau protein expressed in E. coli ; aa. 243-441, contains the four repeated sequences involved in microtubule binding (MTB)
reacts with tau protein and with tau from Alzheimer paired helical filaments; also MAP2; well-suited for gel immunoprecipitation techniques
-
Tau pS214
Biosource Invitrogen
polyclonal
Rabbit
(affinity purified Ig, Dulbecco's PBS, BSA, glycerol, unconjugated)
-
Human
phospho peptide derived from the region of human Tau that contains serine 214
detects tau phosphorylated at serine 204; no cross with non phosphorylated tau
-
FLAG
Sigma Aldrich
polyclonal
IgG
Rabbit
(affinity purified, buffered aqueous solution, IgG, PBS, BSA, azide)
-
Non-specific
immunogen = Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH
recognizes the FLAG epitope located onFLAG fusion proteins; reacts with Nterminal,N-terminal-Met, and C-terminal FLAG fusion proteins
-
GFP (JL-8)
Clontech, a Takara Bio company
monoclonal
IgG2a
Mouse
(liquid, affinity purified, PBS, filter-sterilized)
-
Mammal (various), Microorganism (various)
immunogen = Aequorea victoria green fluorescentprotein (EGFP)
recognizes native and denatured forms of wild-type GFP, GFPuv, EGFP, destabilized EGFP variants, EBFP, EYFP, ECFP, AcGFP, and both N- and C-terminal fusion proteins containing these GFP variants in bacterial and mammalian cell lysates
-
GFP
Clontech, a Takara Bio company
monoclonal
IgG1 ,κ
Mouse
(liquid, affinity purified, Tris-HCl, azide)
-
Mammal (various), Microorganism (various)
immunogen = recomb. full-lengthAequorea victoria green fluorescentprotein (rGFP)
reacts with wild-type GFP, GFPuv, EGFP, destablizedEGFP variants, EBFP, EYFP, ECFP andboth N- and C-terminal fusion proteinscontaining these GFP variants
-