Antibody

Myosin light chain, phos ser19 (MF-20)

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Clonality: monoclonal
Host: Mouse
Isotype: IgG1
Source: Cell Signaling
Cat# 3675 (Mab) and Cat# 3671 (poly, rabbit).
Reference:
Rex CS, 2010
Formulation: mono: liquid, HEPES, BSA, glycerol, azide;<br />poly: liquid, protein-A affinity purified, HEPES, saline, BSA, glycerol, azide;
Reactivity:
Reacts in: Bovine, Pig, Human, Mouse, Rat
plus chicken, D. melanogaster, Xenopus and zebrafish for the rabbit antibody

Immunogen/Epitope

Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2

Specificity

detect endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. These antibodies do not cross-react with the cardiac isoform of myosin light chain 2

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References

Paper Citations

  1. Rex CS, Gavin CF, Rubio MD, Kramar EA, Chen LY, Jia Y, Huganir RL, Muzyczka N, Gall CM, Miller CA, Lynch G, Rumbaugh G. Myosin IIb regulates actin dynamics during synaptic plasticity and memory formation. Neuron. 2010 Aug 26;67(4):603-17. PubMed.

Further Reading

No Available Further Reading