ADAMT10
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, no preservative)
-
Human, Hamster, Mouse
immunogen = synthetic peptide C-RQRPRESYQMGHMRR corresponding to amino acids 735-749 of mouse ADAM10
recognizes the ~98 and ~105 kDa bands corresponding to full length ADAM1O in transfected CHO-K cells, the ~60 kDa cleaved mature protein and the ~30 kDa degradation product
-
ADAMT17 /TACE
EMD Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, PBS, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide (ASFKLQRQN RVDSKETE) corresponding to amino acids 807-823 of human TACE
recognizes the ~130 kDa TACE protein in HeLa and Jurkat cells
-
ADAM17 /TACE (X-135)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein stabilizer)
-
Human
immunogen = full length recombinant TACE of human origin
for detection of TACE; no cross with ADAM8, ADAM9 or ADAM15
-
ADAM17 /TACE (C-15)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = apeptide mapping at the C-terminus of TACE of human origin
for detection of precursor and mature TACE
-
ADAM17 /TACE (H-170)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = amino acids 235-404 mapping within an extracellular domain of TACE of human origin
for detection of TACE
-
ADAM17 /TACE (K-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = apeptide mapping near the C-terminus of TACE of human origin
for detection of precursor and mature TACE
-
ADAM 9 (D64B5)
Cell Signaling
monoclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immungoen = a synthetic peptide corresponding to residues near the carboxy terminus of human ADAM9
detects endogenous levels of total ADAM9 protein
-
ADAM 17
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, PBS, azide, purified)
-
Human, Mouse
Immungoen = a peptide corresponding to amino acids 807-823 of human TACE (differs from mouse, rat by 1 aa.)
detects ADAM17 /TACE
-
ADAM 9
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
Immungoen = a synthetic peptide corresponding to the carboxy terminus of human ADAM9
detects endogenous levels of total ADAM9 protein; , unprocessed and active forms. The antibody does not recognize the carboxy terminally truncated short form of ADAM9
-
APP (22C11)
(Konrad Beyreuther, Center of Molecular Biology, University of Heidelberg, Heidelberg, Germany)PaperReference: Kuhn, 2010
monoclonal
Mouse
-
Human, Monkey, Mouse, Rat
immunogen = a bacterial APP fusion protein (Fd-APP 695), (epitope in ectodomain, at residues 66-81 of APP)
detects a variety of mature as well as immature APP species which differ in their degree of glycosylation
-
APP (7A6)
(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany)PaperReference: Kuhn, 2010
monoclonal
IgG2a
Unknown Host
-
Human, Mouse
immunogen = a peptide comprising amino acids 10–15 of the Aβsequence (YEVHHQ-COOH)
specifically detects APPsα; detects also murine APPsα, but notAPPsβ and APPsβ'
-
APP (4B4)
(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany)PaperReference: Kuhn, 2010
monoclonal
IgG2a
Rat
-
Human
immunogen = a peptide comprising amino acids 11–16 of the Aβsequence (EVHHQK-COOH)
specifically detects APPsα ending in amino acids 15 and 16 (APPs-15 and APPs-16), but does not detect shorter APPs species, including APPsβ' and APPsβ; does not detect full-length APP
-
APP (BAWT)
(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany)Paper, Ulrich et al.Reference: Kuhn, 2010
monoclonal
IgG2a
Mouse
-
Human
immunogen = the peptide ISEVKM derived from the amino acids directly preceding the APPβ-secretase cleavage site
detects APPsβ at the β cleavage site
-
APP C terminal
6687Christian Haass, Ludwig-Maximilians-Universität München, Munich, GermanyPaper, Steiner et al.Reference: Kojro, 2006
polyclonal
Unknown Host
-
Human
immunogen = the 20 most C-terminal amino acids of human APP
human APP C-terminal
-
APP
APP B5 (5313)(Christian Haass, Ludwig-Maximilians-Universität München, Munich, GermanyPaper, Steiner et al.References:Kuhn, 2010Stamer, 2002
polyclonal
Unknown Host
-
Human
immunogen = a sequence in the ectodomain, aa 444-592, of human APP
reactive with APPs, ectodomain
-
alpha APP CTFs
CT15(S Soriano, 2001Paper, JBC online,Dr. Edward Koo, University of California, San Diego),References:Lee, S, 2010Banwait, 2008Rockenstein, 2007Galvan, 2006
polyclonal
Rabbit
-
Human, Mouse
immunogen = syntheticpeptide corresp. to the C-terminal 15 residues of APP (aa 680-695)
APP C-terminal fragments; recognizes the last 15 COOH-terminal amino acid residues of APP695
-
Actin (I-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purifiedAlso available as:sc-1616-R (rabbit)sc-1616 FITC sc-1616 PEsc-1616 TRICT sc-1616 B (biotin)sc-1616 HRPsc-1616 AC Cat# sc1616 AF405, AF488, AF647; Alexa Fluor®)
-
Mammal (various), Dog, Zebra fish, Cow, Horse, Pig, Human, Mouse, Rat, Xenopus, C. elegans, S. cerevisiae, Drosophila
immunogen = peptide mapping at the C-terminus of human actin
broad range of Actin isoforms
-
Aβ 40
anti-amyloid beta FCA3340(Frédéric Checler,Centre National de la Recherche Scientifique),PaperReference: Gandy, 2010
polyclonal
Rabbit
-
Human, Mouse
immunogen = synthetic octapeptide Cys-GLMVGGVV, mimicing the C-terminal end of Aβ 40, coupled to either BSA or KLH
amyloid-beta 40; labels leptomeningeal and cortical arterioles;little or no cross with Aβ 42
-
Aβ 42, C terminal
FCA3542(Frédéric Checler,Centre National de la Recherche Scientifique),PaperReferences:Gandy, 2010Lesné, 2006
polyclonal
Rabbit
(serum)
-
Human, Mouse
immunogen = synthetic octapeptide Cys-MVGGVVIA, mimicing the C-terminal end of Aβ 42, coupled to either BSA or KLH
recognizes amyloid-beta 42; little or no cross with Aβ 40; intensely labels both diffuse and mature lesions
-
Aβ42, murine
JRF/rAβ/2 (Paul Mathews, New York University, School of Medicine,Center for Dementia Research)Research paperReference: Boland, 2010
monoclonal
Mouse
(liquid, horseradish peroxidase conj.)
-
Mouse
immunogen = a peptide corresponding to murine Aβ residues 1–15 (DAEFGHDSGFEVRHQ)
recognizes mouse but not human Aβ1–40 or Aβ1–42
-
Aβ 1-40
JRF/Aβtot/17(Paul Mathews, New York University, School of Medicine,Marc Merken,Johnson & Johnson Pharm. R&D,paper)References:Gandy, 2010Trinchese, 2008Mi, 2007
monoclonal
Mouse
-
Human
immunogen = human Aβ1-40
amyloid-beta, binds within residues 1-15
-
Aβ 1-15
JRF/rAβ1-15/2(Paul Mathews, New York University, School of Medicine,Marc Merken,Johnson & Johnson Pharm. R&D,paper)References:Gandy, 2010
monoclonal
Mouse
-
Mouse
epitope within residues 1-15 of mouse Aβ
binds to amyloid-beta
-
Aβ 40, C-terminus
JRF/cAβ40/10(Paul Mathews, New York University, School of Medicine,Marc Mercken,Johnson & Johnson Pharm. R&D,Paper)References:Boland, 2010Trinchese, 2008Mi, 2007Razmahel, 2002
monoclonal
Mouse
-
Human, Mouse
immunogen = a peptide corresponding to Aβ residues 36–40 (VGGVV)
recognizes the carboxyl terminus of Aβ40; does not detect Aβ1–42 by ELISA or WB, or full-length APP by IP or WB
-
Aβ 42, C-terminal
JRF/cAβ42/26(Marc Mercken,Johnson & Johnson Pharm. R&D,Paper)References:Boland, 2010Trinchese, 2008Mi, 2007Razmahel, 2002
monoclonal
Mouse
-
Human, Mouse
immunogen = the carboxy terminal 10 residues of Ab1-42
amyloid-beta 42, C-terminus
-
Aβ[4-10] and APPβ (W0-2)
(Konrad Beyreuther, Center of Molecular Biology, University of Heidelberg, Heidelberg, Germany)PaperReference: Kuhn, 2010
monoclonal
IgG2a
Mouse
-
Human, Guinea Pig
immunogen = decapeptides overlapping each other by one aminoacid, covering residues 1-42 of Aβ (epitope mapping corr. to amino acid residues 4-10 of human Aβ-peptide)
High affinity to Amyloid peptides Aβ 1-38, Aβ 1-39, Aβ 1-40
-
Aβ
3552(Harald Steiner,Laboratory for Alzheimer’s and Parkinson’s Disease Research, Adolf ButenandtInstitute, Ludwig Maximilians University, Munich, Germany)PaperReference: Kuhn, 2010
polyclonal
Unknown Host
-
Human
immunogen = Aβ1–40
recognizes amyloid-beta
-
Amyloid β1-42 (H31L21)
Biosource Invitrogen
monoclonal
IgG
Rabbit
(liquid, PBS, azide)
-
Human, Mouse
immunogen = peptide (VGGVVIA) corresp. to aa707-713 of P05067
specific to Amyloid β1-42; not Amyloid β1-40, Amyloid β1-43, Amyloid β1-37 or Amyloid β1-38
-
Amyloid β42 (20-1)
Biosource Invitrogen
monoclonal
IgG
Rabbit
(liquid, PBS, azide, protein A purified)
-
Human
immunogen = aa residues from the C-terminus of human amyloid beta A4 protein
specific to Amyloid β42; no cross reaction with Amyloid beta 1-40
-
Aβ
-
monoclonal
(supernat, RIA screened, HPLC purified)
-
-
immunogen = synthetic peptide fragment derived from different regions of amyloid-beta and coupled to a carrier protein
amyloid-beta
-
GFAP (ZCG29)
Invitrogen Corporation
monoclonal
IgG1 ,κ
Mouse
(liquid, tissue culture supernatant, PBS, BSA, azide, concentrate.Also available predilute, ready to use, and 2nd Gen predilute, ready to use)
-
Human, Mouse
immunogen = human brain
reacts with GFAP-containing cells, and labels only the 56 kDa GFAP band from immunoblots; no cross with neurofilament, cytokeratin, vimentin, or desmin; useful in the identification of benign astrocytes and neoplastic cells of glial lineage
-
GFAP
Sigma Aldrich
polyclonal
IgG
Rabbit
(liquid, buffered aqueous solution, IgG fract., PBS, azide)
-
Human, Rat
immunogen = GFAP from human brain
GFAP in astrocytes, glia cells (Bergmann glia), gliomasand other glial cell derived tumors
-
COX-1
Accurate
polyclonal
Goat
-
Mammal (various), Rodent (various), Sheep
COX-1
-
COX-2 (12C4F12)
Molecular Probes Invitrogen
monoclonal
IgG2a ,κ
Mouse
(serum-free medium, purified bybiochemical fractionation, HBS, azide)
-
Human
immunogen = Human Complex IV subunit II, from SDS-PAGE
recognizes COX-2
-
COX-1 (1D6E1A8)
Invitrogen Corporation
monoclonal
IgG2a ,κ
Mouse
(serum-free medium, purified bybiochemical fractionation, HBS, azide)
-
Bovine, Zebra fish, Human, Mouse, Rat, C. elegans
immunogen = Human Complex IV subunit I (COX1)
recognizes COX-1
-
Src, phosY418
Biosource Invitrogen
polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purifiedAlso available Alexa Fluor® 488 for IF, Cat# 44-660A1)
-
Chicken, Human, Mouse
immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine)
detects Src; also reacts with Fyn and Yes
-
Src, phosY529
Biosource Invitrogen
polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purified)
-
Chicken, Human, Mouse, Rat
immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine).
detects Src; not tested for reaction with Fyn and Yes
-
Fyn (15)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid IgG, PBS, azide, gelatinAlso available as sc-434PE for FC, and sc-434AC for IP)
-
Human, Mouse, Rat
epitope mapping between amino acids 85-206 of Fyn p59 of human origin
detects Fyn p59
-
p70 S6 Kinase total (49D7)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase total (49D7)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol.Also available Biotinylated, Cat# 5707)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase total
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding the carboxy terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase phos Thr389 (1A5)
Cell Signaling
monoclonal
IgG2a
Mouse
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr389 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389; also detects p85 S6 kinase when phosphor. at the analogous site (Thr412) and possibly S6KII phosphor. at Thr401
-
MAP2[2a, 2b, 2c] (HM-2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(ascites fluid, azide)
-
Bovine, Chicken, Quail, Human, Mouse, Rat
immunogen = rat brain MAPs
all forms of MAP2; not tubulin, or any other maps; selective labeling of dendritic trees throughout the brain
-
MAP1 light chain LC3 (2G6)
nanoTools Antikörpertechnik GmbH & Co.
monoclonal
IgG1
Mouse
(liquid)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = synthetic peptide conjugated to hemocyanin (epitope at N-Terminus of LC3-B)
recognizes MAP1 light chain component LC-3
-
MAP1 LC3A/B
Cell Signaling
polyclonal
Rabbit
(liquid, protein A and affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues surrounding Gly40 of LC3A/B
detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C.
-
MAP1 LC3B (D11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues near the amino terminus of LC3B
detects endogenous levels of total LC3B protein. Cross-reactivity occur with other LC3 isoforms
-
MAP1 LC3A (D50G8)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues near the amino terminus of LC3A
detects endogenous levels of total LC3A protein Cross-reactivity occur with LC3B
-
GLUT4, C-terminus
Chemicon and Upstate are Millipore
polyclonal
IgG
Rabbit
(liquid, affinity purified, buffer, PBS, azide)
-
Bovine, Pig, Human, Mouse, Rat
immunogen = synthetic peptide corresponding to the C-terminus (amino acids 498-510) of mouse GLUT-4
recognizes GLUT4; ~55 kDa by WB
-
GLUT1
A D I
polyclonal
IgG
Rabbit
(affinity purified, buffer, PBS, BSA, lyophilizedAlso available as unpurified antiserum, GT11-S)
-
Mouse
immunogen = 12-aa peptide mouse GT11, conjugated to KLH; epitope location~ C-terminal, Cytoplasmic domain
recognizes GLUT1
-
Ubiquitin+1, frameshift mutant
Ubi-B1 and Ubi-B2 (Fred W. van Leeuwen, Netherlands Institute forBrain Research, Amsterdam, Netherlands)Paper
polyclonal
Rabbit
(See also R&D systems antibodies MAB703 andAF703)
-
Human
immunogen = synthetic peptides covering YADLRED PDRQDHH PGSGAQ at the C-terminus of Ubiquitin+1
reacts with Ubiquitin+1 and not Ubiquitin
-
Ubiquitin+1, frameshift mutant
(Sungmin Song, Department of Life Science, Kwangju Institute of Science and Technology, Kwangju, Korea)PaperReference: Ko, S, 2010
monoclonal
Rat
(liquid, affinity purified)
-
Human
immunogen = synthetic peptide of the same amino acid sequence as previously described in van Leeuwen et al., 1998
reacts with Ubiquitin+1 and not Ubiquitin
-
EEA1
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues surrounding Gly14 of human EEA1 protein
detects endogenous levels of total EEA1 protein
-
EEA1 (C45B10)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues surrounding Ser70 of human EEA1 protein
detects endogenous levels of total EEA1 protein
-
CI-MPR
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, no preservative)
-
Mouse, Rat
immunogen = a synthetic peptide from the internal part of mouse Mannose 6 Phosphate Receptor (Cation independent) conjugated to an immunogenic carrier protein
detects Cation independent Mannose 6 Phosphate Receptor
-
CI-MPR
Abcam
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, saline, glycerol, azide)
-
Human, Mouse
immunogen = synthetic non-phosphopeptide derived from human Mannose 6 Phosphate Receptor (Cation independent) around the phosphorylation site of serine 2484 (D-D-S-D-E)
detects endogenous levels of total Cation independent Mannose 6 Phosphate Receptor
-
CI-MPR
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
-
Cow, Pig, Human, Mouse
immunogen = full length native protein purified from adult Bovine liver tissue
detects Cation independent Mannose 6 Phosphate Receptor, ~300 kDa by WB
-
Cu/Zn SOD (1G2)
MBL International
monoclonal
IgG1
Mouse
(protein-A purified, no preservative, lyophilized)
-
Human
Immunogen = GST-full length humanSOD1 fusion protein
CuZn/SOD
-
Cu/Zn SOD
MBL International
polyclonal
IgG
Rabbit
(purified, PBS, BSA, glycerol, azide)
-
Human, Mouse, Rat
-
CuZn/SOD
-
SOD1
Assay Designs is Enzo Life Sciences
polyclonal
Rabbit
(liquid, protein-A affinity purified, PBS, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = native rat Cu/Zn SOD-1
recognizes Cu/Zu SOD
-
SOD1
Assay Designs is Enzo Life Sciences
polyclonal
Rabbit
(liquid, protein-A affinity purified, PBS, glycerol, azide)
-
Human, Mouse, Rat, Clam
Immunogen = native human Cu/Zn SOD-1
recognizes Cu/Zu SOD
-
Neuropeptide Y
Immunostar
polyclonal
IgG
Rabbit
(whole serum, azide, lyophilized)
-
Avian (various), Fish (various), Cat, Frog, Human
immunogen = neuropeptide Y; coupled to bovine thyroglobulin (BTg) with glutaraldehyde
specific to NPY; stains paraventricular nucleus, cortex and caudate putamen
-
Somatostatin (YC7)
Chemicon and Upstate are Millipore
monoclonal
IgG2b
Rat
(liquid, culture supernatant, thimerosal)
-
Rabbit, Human, Rat
immunogen = synthetic 1-14 cyclic somatostatin conjugated to bovine thyroglobulin using carbodiimide
reacts with somatostatin. Shows no cross reactivity to enkephalins, other endorphins, substance P or CGRP. Partially cross-reacts with somatostatin fragments
-
Tau TAUY6
Biosource Invitrogen
polyclonal
Rabbit
(liquid, purified Ig, PBS, azide)
-
Human
immunogen = synthetic peptide, corresp. to aa residues 186-201 of human tau protein isoform 6 (GEPPKSGDRSG-pY- SSPG), conj. to KLH
detects both non-phosphor. and p56lck -phosphor. tau
-
Tau TAUY21
Biosource Invitrogen
polyclonal
Mouse
(liquid, purified Ig, PBS, azide)
-
Human
immunogen = synthetic peptide, corresp. to aa residues 21-36 of human tau protein isoform 6 (GDRKDQGG-pY-TMHQDQE), conj. to KLH
detects both non phosphor. and p56lck phosphor. tau
-
Tau, truncated, cleavage site 421/422 (Tau-C3)
Biosource Invitrogen
monoclonal
IgG1 ,κ
Mouse
(affinity purified ascites, PBS, BSA, azide, unconj.)
-
Human, Mouse, Rat
immunogen = KLH- CSSTGSIDMVD, corresp. to the C-terminus of tau truncated at Asp421
recognizes tau when truncated at Asp421; no reaction with full-length tau, or with tau truncated at Glu391 or Ala429
-
Tau (TAU-5)
BD Pharmingen
monoclonal
IgG1
Mouse
(aqueous buffered liquid, supernate, affinity purified, azide)
-
Rat
immunogen = phosphorylation -independent region in the middle of Tau
recognizes both phos. and non phos. forms of Tau
-
Tau pT181
Biosource Invitrogen
kit
Unknown Host
(contains:Hu Tau [pT181]Antibody Bead Concentrate;Hu Tau [pT181]Detection Ab Concentrate;Hu Tau [pT181] Standard)
-
Human
human tau-derived peptide that is phosphorylated at threonine 181
for the measurement of human Tau when phosphorylated at threonine 181 in CSF and tissueculture media
-
Tau (HT7)
Pierce Thermo Scientific
monoclonal
IgG1 κ
Mouse
(frozen liquid, no carrier or preservative, biotin conj.)
-
Bovine, Human
immunogen = human tau, epitope between aa 159 and 163
tau and PHF tau tau between aa 159-163
-
Tau-1 (PC1C6)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(purified Ig, phosphate buffered, lyophilized)
-
Bovine, Human, Rat
immunogen = purified denaturated bovine MAPs (epitope at or near a phosphorylated site)
binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE); there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(purified Ig, buffered aqueous solution, PBS, BSA, azide, FITC)
-
Various species, Mouse, Rat
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host
-
GFP (C163)
Zymed/ Invitrogen
monoclonal
IgG1 ,κ
Mouse
(liquid, affinity purified, PBS, azide)
-
Non-specific
immunogen = purified recombinant GFP protein from the jellyfish Aequorea victoria
reacts with GFP
-
GFP
Vector Labs
polyclonal
Goat
(liquid, affinity purified, HEPES, sodium sulfate, thimerosal)
-
Non-specific
-
for direct detection of GFP fusion proteins on western blots usingchromogenic or chemiluminescent peroxidase substrates
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(affinity isolated, buffered aqueous solution, phosphate, glycerol, no antimicrobial preservatives)
-
Various species
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(purified IgG, buffered aqueous solution, phosphate, saline, azide)
-
Various species
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent
-
GFP (11E5)
MP Biomedicals
monoclonal
IgG1
Mouse
(purified from the supernatant of mouse hybridomas; store at -80°C)
-
Non-specific
immunogen = green fluorescent protein (GFP)
allows detection of as little as 10 ng of GFP using colorimetric methods, and will detect picogram quantities with chemiluminescentdetection assays
-
GFP
Molecular Probes Invitrogen
polyclonal
IgG
Rabbit
(liquid, IgG fraction, Alexa Fluor® 488 conjugateAlso available asAlexa Fluor® 555, A31851;Alexa Fluor® 594, A21312;Alexa Fluor® 647, A31852)
-
Non-specific
-
reacts with GFP
-
GFP
Molecular Probes Invitrogen
polyclonal
IgG
Rabbit
(liquid, antiserum, thimerosal, unonjugated)
-
Non-specific
immunogen = GFP isolated directly from the jellyfish Aequorea victoria
reacts with GFP
-
GFP (3E6)
Molecular Probes Invitrogen
monoclonal
IgG2a
Mouse
(purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA, unonjugated, lyophilized)
-
Non-specific
immunogen = green fluorescent protein (GFP) that was isolated directly from Aequorea victoria
specific for GFP
-
GFP (11E5)
Molecular Probes Invitrogen
monoclonal
IgG1
Mouse
(purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA, unonjugated, lyophilized)
-
Non-specific
immunogen = green fluorescent protein (GFP) that was isolated directly from Aequorea victoria
reacts with GFP
-
GFP
Molecular Probes Invitrogen
polyclonal
IgG
Rabbit
(liquid, IgG fraction, PBS, azide)
-
Non-specific
immunogen = green fluorescent protein (GFP) that was isolated directly from A. victoria and purified by ion-exchange
reacts with GFP
-
GFP (3E6)
MP Biomedicals
monoclonal
IgG2a
Mouse
(purified from the supernatant of mouse hybridomas; store at -80°C)
-
Non-specific
immunogen = green fluorescent protein (GFP)
highly specific for GFPs in both bacterial and mammalian cell extracts; allows purification of GFP and GFP-fusion proteins with either protein A or protein G
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