The Antibodies database was archived in December 2022 and is no longer being updated. The Antibodies database is being supplanted by the AlzAntibodies database, which aims to provide detailed descriptions of selected antibodies.

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Antibodies

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	Name	Source	Clonality & Isotype	Host & Formulation	Method	Reactivity	Immunogen/ Epitope	Specificity	Availability
Akt and Serine/Threonine Kinases From manufacturers (22)
	Akt (8F4)	tebu-bio	monoclonal IgG1,κ	Mouse (PBS, azide, PEG, sucrose, purified)	-	Human, Mouse, Rat	Immunogen = peptide corresponding to the C-terminus aa 466-480 of Akt coupled to KLH	Akt/PKB	-
	Akt phos Ser473 (M89-61)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, aqueous buffered solution, BSA, azide, purified, AlexaFluor® 647)	-	Human, Mouse	Immunogen = phosphorylated human Akt1 pS473 peptide	Akt phosphorylated at Ser473	-
	Akt phos Ser473 (M89-61)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, affinity purified, buffered, BSA, azide, conj. PE)	-	Human, Mouse	immunogen = phosphorylated human Akt1 pS473 peptide	recognizes Akt phosphorylated at Ser473; phosphorylationsite is shared by all three isoforms of Akt	-
	Akt phos Ser473 (M89-61)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, aqueous buffered solution, BSA, azide, purified)	-	Cow, Human, Mouse, Rat	Immunogen = phosphorylated human Akt1 pS473 peptide	Akt phosphorylated at Ser473	-
	Akt phos Ser473 (M89-61)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, aqueous buffered solution, BSA, azide, purified, AlexaFluor® 488)	-	Human, Mouse	Immunogen = phosphorylated human Akt1 pS473 peptide	Akt phosphorylated at Ser473	-
	Akt1/PKBa (55)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, affinity purified, buffered, BSA, azide, conj. AlexaFluor® 647)	-	Dog, Human, Mouse, Rat	immunogen = human Akt1 recombinant protein	recognizes Akt 1 regardless of phosphorylation status	-
	Akt1/PKBa (55)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, affinity purified, buffered, BSA, azide, conj. PE)	-	Dog, Human, Mouse, Rat	immunogen = human Akt1 recombinant protein	recognizes Akt 1 regardless of phosphorylation status	-
	Akt1/PKBa (55)	BD Pharmingen	monoclonal IgG1, κ	Mouse (liquid, affinity purified, buffered, BSA, azide, conj. AlexaFluor® 488)	-	Dog, Human, Mouse, Rat	immunogen = human Akt1 recombinant protein	recognizes Akt 1 regardless of phosphorylation status	-
	Akt	Sigma Aldrich	kit	(capture and detection ab biotinylated)	-	Human, Mouse, Rat	Immunogen = full length Akt/PKB	Total Akt/PKB. Does not cross-react with p38 MAPK, p42 Erk1, p42 Erk2, JNK 1, human/rat insulin receptor, or human EGFR	-
	Akt1	Sigma Aldrich	polyclonal	Rabbit (lyophilized, PBS, sucrose)	-	Human	immunogen = synthetic peptide corresp. to a region of human AKT1 with an internal ID of P01102	Akt1	-
	Akt2	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human	immunogen = synthetic peptide corresponding to amino acids 121-135 of human AKT2	Akt2	-
	Akt2	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human	immunogen = synthetic peptide corresponding to amino acids 454-469 of human AKT2	Akt2	-
	Akt3	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human	immunogen = synthetic peptide corresponding to amino acids 119-135 of human AKT3	Akt3	-
	Akt3	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human	immunogen = synthetic peptide corresponding to amino acids 401-415 of human AKT3	Akt3	-
	Akt1 phos Ser473	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human, Mouse, Rat	immunogen = synthetic phospho-peptide corresp. to the pSer473 region of human PKBα (aa461-477).	Akt1/PKBα phos-phorylated Ser473	-
	Akt1 phos Thr308	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Human, Mouse, Rat	immunogen = synthetic phospho-peptide corresp. to the pThr308 region of human PKBα (aa 301-315)	Akt1/PKBα phos-phorylated Thr308	-
	Akt1 (2E11)	Sigma Aldrich	monoclonal IgG2a, κ	Rabbit (liquid, PBS, purified)	-	Human, Mouse, Rat	immunogen = AKT1 (AAH00479, aa 1-481) full length recombinant protein with GST tag. MW of the GST tag alone is 26 kDa	Akt1	-
	Akt2 (1B3)	Sigma Aldrich	monoclonal IgG1, κ	Rabbit (liquid, PBS, purified)	-	Human	immunogen = AKT2 (AAA58364, a.a. 100-189) partial recombinant protein with GST tag. MW of the GST t.ag alone is 26 kDa	Akt2	-
	Akt3 (6E10)	Sigma Aldrich	monoclonal IgG2a, κ	Rabbit (liquid, PBS, purified)	-	Human	immunogen = AKT3 (AAD29089, a.a. 100-189) partial recombinant protein with GST tag. MW of the GST tag alone is 26 kDa	Akt3	-
	Akt1 paired S473	Spring Bioscience	polyclonal	Rabbit (liquid, PBS, BSA, azide, affinity purified)	-	Human	Immunogen = synthetic peptide surrounding Ser473 which corresponds to the C-terminus of human Akt1 protein	Akt1 paired S473 (60kDa), non-phospho-specific	-
	Akt1 phos Ser473	Spring Bioscience	polyclonal	Rabbit (liquid, PBS, BSA, azide, affinity purified)	-	Human	Immunogen = synthetic peptide corresp. to the C-terminus of human Akt1/PKBα at the phosphorylated site of Serine 473	Akt1/PKB phosphorylated Ser473	-
	Akt1 phos Ser473	Spring Bioscience	polyclonal	Rabbit (liquid, PBS, BSA, azide, affinity purified; prediluted, RTU)	-	Human	Immunogen = synthetic peptide corresp. to the C-terminus of human Akt1/PKBα at the phosphorylated site of Serine 473	Akt1/PKB phosphorylated Ser473	-
Amyloid-β From manufacturers (12)
	β-Amyloid [1-40]	Sigma Aldrich	polyclonal	Rabbit (serum, delipidized, azide)	-	Human	immunogen = β-Amyloid [1-40], corr. to APP(672-711), conj. to BSA	β-Amyloid [1-40] in plaques of Alzheimer disease patients; no staining of normal brain tissue	-
	β-Amyloid [22-35]	Sigma Aldrich	polyclonal	Rabbit (buffered aqueous solution, affinity purified, PBS, BSA, azide)	-	Human, Mouse, Rat	immunogen = synthetic peptide corresp. to amino acids 22-35 of human β-amyloid (1-40) fragment, conjugated to KLH	recognizes β-amyloid(22-35), β-amyloid (25-35), β-amyloid (1-40) andβ-amyloid (1-42) and does not react with β-amyloid(32-35) and β-amyloid (35-25)	-
	β-Amyloid 42	Sigma Aldrich	polyclonal	Rabbit (serum, affinity purified, phosphate buffer)	-	Human, Mouse, Rat	immunogen = synthetic peptide of approximately 10 aa residues at the C-terminus of Aβ 42.	Aβ cleavage site 42 with free carboxyl group at C terminus; not Aβ40, Aβ 43 or APP	-
	β-Amyloid 43	Sigma Aldrich	polyclonal	Rabbit (serum, affinity purified, phosphate buffer)	-	Human, Mouse	immunogen = synthetic peptide of approximately 7-10 aa residues at the C-terminus of Aβ 43.	Aβ cleavage site 43; cleavage sitespecific and its binding to the Ab peptide requires the presence of the free carboxyl group at the C- terminus of the peptide; not full length Aβ40 or Aβ 42	-
	β-Amyloid, 1-12 (BAM-10)	Sigma Aldrich	monoclonal IgG1	Mouse (liquid, PBS, azide, purified)	-	Human	immunogen = synthetic β-amyloid peptide (1-40), conj. to KLH. (epitope within amino acid residues 1-12)	β-amyloid protein; epitope resides within aa 1-12	-
	β-Amyloid (NAB228)	Sigma Aldrich	monoclonal IgG2a	Mouse (liquid, PBS, azide, purified)	-	Human	immunogen = synthetic peptide corr. to aa 1-11 of human β-amyloid, conj. to KLH	monoclonal recognizes β-amyloid peptide; full-length amyloid precursor protein (APP), soluble-APP (sAPPβ' and sAPPα), C99 cleavage form, and Aβ (1-40/42), but not soluble-APP form sAPPβ.	-
	β-Amyloid [1-17] (6E10)	Sigma Aldrich	monoclonal IgG1	Mouse (ascites, protein-G, PBS, thimerosal)	-	Human, Mouse	immunogen = synthetic peptide corr. to aa 1-17 of β-amyloid, with Glu substituted at position 11, conjugated to KLH. Epitope at aa 1-12	monoclonal recognizes aa 1-17 of human amyloid β peptide; also recognizes the precursor forms and abnormally processed isoforms	-
	β-Amyloid [13-28] (BAM90.1)	Sigma Aldrich	monoclonal IgG1	Mouse (liquid, PBS, purified, sterile filtered)	-	Human	immunogen = synthetic peptide corr. to aa 13-28 of human β-amyloid, conjugated to KLH; Epitope resides within aa 20-23	monoclonal detects β-Amyloid peptide	-
	β-Amyloid [1-42]	Sigma Aldrich	kit	Unknown Host (solid phase sandwich, wells coated with monoclonal specific for NH2 terminus of hAβ.detection antibody, A1728, producedin rabbit, which recognizes human Aß42)	-	Human		both natural and synthetic forms of β-Amyloid 42; not β-Amyloid 40 or β-Amyloid 43	-
	Amyloid β	Chemicon and Upstate are Millipore	polyclonal	Rabbit (liquid, affinity purified Ig, PBS, no preservative)	-	Rodent (various)	immunogen = rodent beta amyloid peptide, aa 3-16	specific to Amyloid β, all isoforms	-
	Amyloid β	Chemicon and Upstate are Millipore	polyclonal	Rabbit (purified Ig, PBS, no preserve)	-	Rodent (various)	immunogen = rodent beta amyloid peptide	specific to Amyloid β, all isoforms	-
	Amyloid β [1-42]	Biosource Invitrogen	kit	Unknown Host (high sensitivity kit, pre-coated plate, standard, detector antibody, anti-IgG-HRP, diluents, wash buffer, chromogen, stop solution)	-	Human	-	detects natural and synthetic Human Ab 42 in tissue culture medium, tissue homogenate, cerebrospinal fluid (CSF) samples.	-
CREB, cAMP, CBP From manufacturers (1)
	CREB phos Ser133	Upstate and Chemicon are Millipore	polyclonal IgG	Rabbit (liquid, PBS, BSA, azide, Protein A/affinity purified)	-	Human, Hamster, Mouse, Rat	Immunogen = synthetic phospho-peptide corresponding to residues 123-136 of rat CREB coupled to KLH	recognizes 43 kDa CREB phosphorylated at Ser133. May also recognize phosphorylated CREM (p30) and ATF-1 (p38)	-
Cell Cycle From manufacturers (2)
	CDC42 (44)	BD Transduction	monoclonal IgG1	Mouse (ascites, affinity purified, aqueous buffered solution, BSA, glycerol, azide)	-	Dog, Human, Mouse, Rat	Immunogen = human CDC42, aa. 1-91	recognizes CDC42	-
	Rac1	Pierce Thermo Scientific	monoclonal IgG2b	Mouse (GST-human Pak1-PBD, anti-Rac1 antibody, GTPγS, GTP)	-	Dog, Chicken, Human, Mouse, Rat	-	amount of antibody required to efficiently detect Rac1 in 20 μg NIH3T3 whole celllysate by Western blo	-
Cell Death From manufacturers (1)
	cytochrome c (H-104)	Santa Cruz	polyclonal IgG	Rabbit (liquid IgG, PBS, azide, gelatin.Also available as agarose conj., sc-7159 AC, for IP)	-	Horse, Human, Mouse, Rat	immunogen = aminoacids 1-104 representing full length cytochrome c of horse origin	detects Cytochrome c	-
DNA Damage & Repair From manufacturers (1)
	PARP [H-250]	Santa Cruz	polyclonal IgG	Rabbit (liquid, PBS, azide, gelatin)	-	Human, Mouse, Rat	immunogen = aa. 764-1014 mapping at the C-terminus of human PARP	reacts with PARP-1 and to a lesser extent with PARP-2	-
F4/80 From manufacturers (5)
	F4/80 (3H2113)	Santa Cruz	monoclonal IgG2b	Rat (liquid, PBS, azide, gelatin)	-	Mouse	immunogen = thioglycollate stimulated peritoneal macrophages from C57/BL mice	detects F4/80	-
	F4/80 (6A404)	Santa Cruz	monoclonal IgG2b	Rat (liquid, PBS, azide, gelatin)	-	Mouse	immunogen = thioglycollate stimulated peritoneal mouse macrophages	detects F4/80	-
	F4/80 (6A545)	Santa Cruz	monoclonal IgG2a	Rat (liquid, PBS, azide, gelatin)	-	Human, Mouse	immunogen = raised against cultured bone marrow-derived mouse macrophages	detects F4/80/EMR1	-
	F4/80 (3C137)	Santa Cruz	monoclonal IgG2b	Rat (liquid, PBS, azide, gelatin)	-	Mouse	immunogen = thioglycollate stimulated peritoneal mouse macrophages	detects F4/80/EMR1	-
	F4/80 (CI:A3-1)	Santa Cruz	monoclonal IgG2b	Rat (liquid, PBS, azide, gelatin)	-	Mouse	immunogen = thioglycollate stimulated peritoneal mouse macrophages	detects F4/80	-
GSK-3α & GSK-3β From manufacturers (1)
	GSK3β	Zymed/ Invitrogen	monoclonal	Mouse (liquid, affinity purified, unconjugated)	-	Human, Mouse, Rat	-	recognizes the expressed product of the GSK3B gene	-
Glutamate Receptor & Related From manufacturers (6)
	GluR1	Upstate and Chemicon are Millipore	polyclonal IgG	Rabbit (liquid, protein-A purified, Tris-Glycine, saline, glycerol, azide)	-	Rat	Immunogen = KLH-conjugated, synthetic peptide (C-RDHTR VDWKRPK) corresp. to aa. 276-287 of rat glutamate receptor 1 (GluR1), with a cysteine added for conjugation	detects GluR1; ~106 kDa	-
	GluR1 phos Ser845	Chemicon and Upstate are Millipore	polyclonal	Rabbit (liquid, Hepes, NaCl, BSA, glycerol, affinity purified)	-	Rat	Immunogen = synthetic peptide corresponding to aa surrounding the phosphoSer845 of rat GluR1	GluR1 phosphorylated Ser845 (100kDa)	-
	GluR1 (EPR2148)	Upstate and Chemicon are Millipore	monoclonal IgG	Rabbit (liquid, unpurified, Tris-Glycine, saline, glycerol, azide, BSA)	-	Human, Mouse, Rat	Immunogen = phospho-specific peptide corresponding to residues surrounding Ser845 of human GluR1	recognizes GluR1 phosphorylated on Ser845; ~106 kDa	-
	NMDAR2B (13)	BD Transduction	monoclonal IgG2b	Mouse (liquid, BSA, glycerol, azide)	-	Human, Mouse, Rat	Immunogen = aa 892-1051 of rat NMDAR2B	detects NMDAR2B	-
	NMDAR1 (54.1)	BD Transduction	monoclonal IgG2a	Mouse (liquid, purified, aqueous buffered solution, azide)	-	Human, Monkey, Rat	Immunogen = rat NMDAR1 aa 660-811, near the C-terminus	detects NMDAR1; no cross with NMDAR2-5 receptors	-
	NMDAR2A (5)	BD Transduction	monoclonal IgG1	Mouse (liquid, affinity purified, BSA, glycerol, azide)	-	Mouse, Rat	Immunogen = mouse NMDAR2A aa 1093-1214 at C-terminus	detects NMDAR2A	-
Heat Shock Proteins From labs (1)
	Hsp90 (4F3)	DO Toft,Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MNPaper	monoclonal	Mouse	-	Chicken, Human	-	hsp90	-
Kinases From manufacturers (1)
	EphA4	Upstate and Chemicon are Millipore	polyclonal	Rabbit (liquid, (NH4)SO2 precipitated rabbitIgG, glycerol, BSA, azide)	-	Mouse, Rat	immunogen = GST EphA4 fusion protein corresp. to the SAM domain and C-terminal tail(amino acids 909 to 987) of mouse EphA4.	Recognizes EphA4 tyrosine kinasereceptor at 120kDa, and degradation products at95kDa and 50kDa	-
Neural Progenitors From manufacturers (4)
	doublecortin	Abcam	polyclonal IgG	Rabbit (liquid, BSA, PBS, azide, affinity purified)	-	Cat, Chicken, Quail, Human, Mouse, Rat, Rhesus	Immunogen = synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Doublecortin	recognizes doublecortin	-
	Nestin	Santa Cruz	polyclonal IgG	Goat (liquid, affinity purified, PBS, azide, gelatin)	-	Human	immunogen = a peptide mapping at the C-terminus of nestin of human origin	recognizes human nestin protein, 200-220 kDa	-
	Nestin (H-95)	Santa Cruz	polyclonal IgG	Rabbit (liquid, PBS, azide, gelatinAlso agarose conjugate for IP, sc-20978 AC)	-	Human, Mouse, Rat	immunogen = amino acids 235-320 of nestin of human origin	recognizes nestin protein, 200-220 kDa	-
	Neurogenin 2	Abcam	polyclonal IgG	Rabbit (liquid, PBS, azide, protein-G affinity purified)	-	Human, Mouse	immunogen = KLH conjugated synthetic peptide (10-30 aa in length) at the C-termimal of last 50 aa. of human Neurogenin 2	detects Neurogenin 2, band around 25 kDa	-
Neurofilaments From manufacturers (1)
	Neurofilament 160/200 kD (MIG-N18)	Pierce Thermo Scientific	monoclonal IgG1κ	Mouse (liquid, ascites, azide)	-	Human	phosphory- lated epitope on the 160- and 200-kD polypeptidesof purified human neurofilament	Human neurofilament	-
Neuronal Development From manufacturers (1)
	PSA-NCAM (2-2B)	Chemicon and Upstate are Millipore	monoclonal IgM	Mouse (liquid, ascites, glycerol, no preservative)	-	Human, Mouse, Rat	Immunogen = Viable Meningococcus group B (strain 355)	Reacts with alpha 2-8 linked neuraminic acid (NeuAc-alpha 2-8) n with n >10 (this polymer is usually termed polysialic acid --PSA). Works well on all species expressing PSA-NCAM	-
Neuronal Markers From manufacturers (1)
	NeuN (A60)	Chemicon and Upstate are Millipore	monoclonal	Mouse (liquid, PBS, stabilizers, Tween 20, ProClin 300, prediluted, ready to use)	-	Avian (various), Primate (various), Chicken, Salamander, Various vertebrate, Ferret, Pig, Human, Mouse, Rat	immunogen = purified cell nuclei from mouse brain	reacts with NeuN	-
Oligodendrocyte Markers/Myelin From manufacturers (3)
	Chondroitin Sulfate Proteoglycan (9.2.27)	Chemicon and Upstate are Millipore	monoclonal IgG2a	Mouse (liquid, protein-A purified, PBS, saline, azide)	-	Human	immunogen = human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™	recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa	-
	NG2, Chondroitin Sulfate Proteoglycan	Chemicon and Upstate are Millipore	polyclonal	Rabbit (liquid, PBS, azide, protein-A affinity purified)	-	Human, Monkey, Mouse, Rat	immunogen = immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat	identifies both the intact proteoglycan and the core protein by Western blot and ELISA	-
	O1 (O1)	R&D Systems	monoclonal IgM	Mouse (filtered solution in PBS, trehalose, lyophilized)	-	Chicken, Human, Mouse, Rat	immunogen = white matter of corpus callosum from bovine brain	recognizes Oligodendrocyte cell surface marker O1	-
PSD95 From manufacturers (3)
	PSD-95 /SAP90 (6G6-1C9)	Pierce Thermo Scientific	monoclonal IgG2a	Mouse (liquid, purified, PBS, azide)	-	Rat	immunogen = purified recombinant rat PSD-95	detects ~95 kDa PSD protein	-
	PSD-95 /SAP90 (7E3-1B8)	Pierce Thermo Scientific	monoclonal IgG2a	Mouse (liquid, purified, PBS, azide)	-	Mouse, Rat	immunogen = purified recombinant rat PSD-95	detects ~95 kDa PSD protein	-
	PSD-95 /SAP90	Pierce Thermo Scientific	polyclonal IgG	Rabbit (liquid, affinity purified, HEPES, saline, BSA, glycerol)	-	Human, Mouse, Rat	immunogen = peptide corresp. to amino acid residues from the N-terminal region of rat PSD-95, conj. to KLH	recognizes ~95 kDa PSD protein	-
Phosphorylation Related From manufacturers (1)
	phospho-tyrosine (PY20)	Santa Cruz	monoclonal IgG2b	Rabbit (PBS, azide, gelatinAvailable for FC as either phycoerythrin (sc-508 PE) or fluorescein (sc-508 FITC).Available for IP as agarose conjugate (sc-508 AC)Available for WB as sc-508 HRPAvailable for FC and IF as Alexa Fluor® 405 (sc-508 AF405), Alexa Fluor® 488 (sc-508AF488) or Alexa Fluor® 647 (sc-508 AF647))	-	Non-specific	-	detects phosphorylated tyrosine residues	-
Post-synaptic proteins From manufacturers (2)
	Homer, N-terminal	Abcam	polyclonal IgG	Rabbit (liquid, purified, PBS, azide)	-	Human	immunogen = synthetic peptide selected from the N terminal region of human Homer conjugated to KLH	detects homer, in cell junction, synapse, post synaptic cell membrane, post synaptic density; band of approximately 40 kDa	-
	Homer, 1b+1c	Abcam	polyclonal IgG	Rabbit (liquid, whole antiserum, no preservative)	-	Human, Mouse, Rat	immunogen = synthetic peptide from the internal part of human Homer (1b+1c) conjugated to an immunogenic carrier protein	detects Homer, 1b+1c, internal part	-
Synaptic Vesicle Fusion From manufacturers (2)
	Bassoon	Synaptic Systems	polyclonal	Guinea Pig (lyophilized, antiserum)	-	Mouse, Rat	immunogen = recombinant protein containing the 330 C-terminal aa of rat bassoon	detects bassoon	-
	Bassoon (4E15)	US Biological	monoclonal IgG2a	Mouse (liquid, PBS, azide, glycerol, protein G affinity purified)	-	Rat	immunogen = recombinant rat bassoon fragment	detects bassoon (~400 kDa, additional bands between 97 and 400kD are also identified and are reported to be proteolytic degradation products of bassoon )	-
Tau From labs (2)
	Tau	BR189(M Goedert, Medical Research Council, Laboratory of Molecular Biology, Cambridge, England)Paper, Neuron. 1989 Oct;3(4):519-26ReferencesClavaguera, 2009	polyclonal	Unknown Host	-	Human	immunogen includes amino acids 76-87 of human tau	specific for tau isoforms with two N‑terminal inserts (the amino-terminal 29- and 58-amino acid inserts of tau)	-
	Tau	MT1(M Goedert, Medical Research Council, Laboratory of Molecular Biology, Cambridge, England)Paper, J Neurosci. November 1, 2002ReferencesClavaguera, 2009	polyclonal	Rabbit	-	Mouse	immunogen = synthetic peptide (ARVASKD RTGNDEK), residues 114-127 of the longest isoform of mouse brain tau	recognizes the three murine brain tau isoforms but fails to recognize human tau; phosphorylation independent; diffuse neuropil staining	-
Tau From manufacturers (8)
	Tau, N-terminal	RDI /Fitzgerald	polyclonal	Goat (neat serum, azide)	-	Human	antigen = synthetic peptide corresp. to aa. 1-16 (MAEPRQEF EVMEDHAG)	detects tau	-
	NeurofibrillaryTangles (MIG-T4 or NFT200)	Pierce Thermo Scientific	monoclonal	Mouse (supernatant, azide)	-	Human		binds tangles, does not react with tau protein	-
	Tau (AT100)	Pierce Thermo Scientific	monoclonal IgG1κ	Mouse (biotin, no carrier or preservative)	-	Human	around phosphorylated Thr212 and Ser214	PHF tau with phosphorylated Thr212 and Ser214, not normal tau, or biopsy, autopsy or fetal tau.	-
	Tau (AT180)	Pierce Thermo Scientific	monoclonal IgG1κ	Mouse (biotin, no carrier or preservative)	-	Human	around phosphorylated Thr231	PHF tau and tamgles with phosphorylated Thr231, weakly with normal tau	-
	Tau (BT2)	Pierce Thermo Scientific	monoclonal IgG1κ	Mouse (purified, no carrier or preservative)	-	Bovine, Human, Monkey, Rat	between aa 194 and 198	tau between aa 194-198, but not PHF tau	-
	Tau (BT2)	Pierce Thermo Scientific	monoclonal IgG1κ	Mouse (biotin, no carrier or preservative)	-	Bovine, Primate (various), Human, Rat	between aa 194 and 198	tau between aa 194-198, but not PHF tau	-
	Tau	Pierce Thermo Scientific	polyclonal IgG	Sheep (serum, purified, PBS, no carrier or preservative, frozen liquid)	-	Bovine, Human, Rat	immunogen = purified human tau	recognizes normal tau from brain, and PHF tau	-
	Tau (BT2)	RDI /Fitzgerald	monoclonal IgG1,κ	Mouse (purified ascites, PBS, BSA, azide)	-	Rabbit, Human	immunogen = recombinant human VEGF121	detects tau	-
Transcription Factors From manufacturers (5)
	Rho A (F-1)	Santa Cruz	monoclonal IgG2b	Mouse (PBS, azide, gelatin.Available for Gel Supershift and ChIP,sc-166399 X)	-	Human, Mouse, Rat	(epitope mappingbetween amino acids 116-140 within an internal region of Rho A of humanorigin)	detects Rho A; 24 kDa	-
	Rho A (26C4)	Santa Cruz	monoclonal IgG2b	Mouse (PBS, azide, gelatin.Available for FC as fluorescein (sc-418 FITC) and phycoerythrin (sc-418 PE)Available fo IF as fluorescein (sc-418 FITC) or rhodamine (sc-418 TRITC)Available for FC or IF as Alexa Fluor® 405 (sc-418 AF405), Alexa Fluor® 488 (sc-418AF488) or Alexa Fluor® 647 (sc-418 AF647))	-	Human, Mouse, Rat	(epitope mappingcorresponding to amino acids 120-150 of Rho A of human origin)	detects Rho A; 24 kDa	-
	Dlx2	Chemicon and Upstate are Millipore	polyclonal	Rabbit (affinity purified, precipitated antibody in solution of 50% saturated ammonium sulfate and PBS, with no preservatives)	-	Human, Mouse	immunogen = synthetic peptide, amino acids 24-39 from human Dlx 2	recognizes Dlx 2 (Distalless (DII) family), a homeodomain transcription factor.	-
	Dlx2	Chemicon and Upstate are Millipore	polyclonal	Rabbit (affinity purified, liquid, PBS, azide)	-	Mouse	immunogen = synthetic peptide	recognizes Dlx 2 (Distalless (DII) family), at ~35 kDa on mouse brain lysate	-
	ASH1	Chemicon and Upstate are Millipore	polyclonal	Rabbit (liquid, PBS, azide, affinity purified)	-	Mouse	immunogen = synthetic peptide	detects ASH1 (MASH1, mammalian achaete-scute homolog 1), band at ~30 kDa	-
Wnt signaling From manufacturers (2)
	WNT3	Zymed/ Invitrogen	polyclonal	Rabbit (antiserum, affinity purified, PBS, azide)	-	Mouse	immunogen = synthetic peptide derived from an internal region of the human WNT3 protein	reacts with mouse WNT3 protein, a band at ~40 kDa; two additional non-specific bands at ~50 kDa and ~60 kDa are observed in both WNT3-transfected and vector-only cell lysates	-
	frizzled (H-300)	Santa Cruz	polyclonal IgG	Goat (liquid, PBS, azide, gelatin)	-	Human, Mouse, Rat	immunogen = amino acids 301-400 of frizzled of human origin	detects frizzled proteins 1-10	-
β-Catenin related From manufacturers (3)
	Beta-Catenin	Chemicon and Upstate are Millipore	polyclonal	Rabbit (serum, liquid, no preservative)	-	Dog, Human, Mouse, Rat	immunogen = recombinant GST-fusion protein (full length β-Catenin)	β-Catenin	-
	Beta-Catenin	Sigma Aldrich	polyclonal	Rabbit (serum, azide, delipidized)	-	Bovine, Human	immunogen = a peptide corresp. to aa 768-781 of human or mouse β-Catenin	aa. 768-781 of β-Catenin, not aa. 890-901 of α-Catenin or alpha-catenin or gamma-catenin	-
	β-Catenin phos Ser33/37	Zymed/ Invitrogen	polyclonal	Rabbit (liquid, affinity purified, HEPES, saline, BSA, glycerol)	-	Human	immunogen = phosphopeptide corresponding to amino acid residues surrounding the phosphoserines 33 and 37 of human β-catenin	specific for the ~83 kDa β-catenin phosphorylated at serines 33 and 37	-
γ-secretase/ Presenilins From manufacturers (9)
	Nicastrin (ab-2)	Sigma Aldrich	polyclonal	Rabbit (buffered aqueous solution, affinity isolated, PBS, azide)	-	Human, Mouse, Rat	immunogen = an 18 amino acid peptide from near the center of human Nicastrin	detects Nicastrin	-
	Nicastrin (ab-1)	Sigma Aldrich	polyclonal	Rabbit (buffered aqueous solution , IgG fract, PBS, azide)	-	Human, Mouse, Rat	immunogen = a 17 amino acid peptide from near the C-terminus of human Nicastrin	detects Nicastrin	-
	Presenilin-1	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide)	-	Mouse, Rat	immunogen = highly purified peptide D P E A Q R R V P K N P K Y corresp. to aa 303-316 from the loop region of mouse presenilin 1	detects presenilin 1 (dimer at 98kDa and endo-proteolytic frag at 21kDa)	-
	Presenilin-2	Calbiochem EMD Biosciences	polyclonal IgG	Rabbit (liquid, affinity purified, PBS, azide, gelatin)	-	Human, Mouse, Rat	immunogen = aa 7-24 from the N-terminus of human PS2	detects N-terminal proteolytic fragment at 28 kDa in WB	-
	Presenilin-2, loop domain	Calbiochem EMD Biosciences	polyclonal IgG	Rabbit (liquid, undiluted serum, no preservative)	-	Human, Mouse	immunogen = recomb. protein containing the loop domain (aa 269-394) of human presenilin-2 fusedto GST	detects full-lengthpresenilin 2 and its C-terminal fragments	-
	Presenilin-1	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide, gelatin)	-	Mouse, Rat	immunogen = highly purified peptide D S Q E R Q Q Q H D R Q R L D N corresp. to aa 31-46 from the N-terminal region of mouse presenilin 1	detects presenilin 1 (dimer at 98kDa and enodproteolytic frag at 30kDa)	-
	Presenilin-1	Sigma Aldrich	polyclonal	Rabbit (liquid, buffered aqueous solution, affinity isolated, PBS, azide)	-	Human, Mouse, Rat	immunogen = a 23 amino acid peptide from near the carboxy-terminus of human presenilin1	detects presenilin 1	-
	Presenilin-1, C-Terminal	Sigma Aldrich	polyclonal IgG	Rabbit (liquid, PBS, azide, purified)	-	Human, Mouse, Rat, Xenopus	immunogen = synthetic peptide (K-D Y L V Q P F M D Q L A F H Q F Y I) corresp. to C-terminal of human presenilin 1 (aa 450-467 with N-terminal added lysine), conj. to KLH	detects presenilin 1, 48 kDa (and a degredation product at ~18kDa); stains neurite plaques in AD brain	-
	Presenilin-1, NT	Sigma Aldrich	polyclonal	Goat (liquid, whole serum, azide)	-	Human	immunogen = purified peptide corresp. to aa 14-33 of presenilin 1	recognizes presenilin 1	-