α-Actin smooth muscle
Anaspec
polyclonal
Rabbit
(liquid, PBS, BSA, azide, conj. Hilyte Fluor™ 405-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresponding to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
Rabbit
(liquid, PBS, BSA, azide, conj. Hilyte Fluor™ 594-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. biotin)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. TAMR)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. FAM)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AMCA)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. FITC)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. HiLyte Fluor™ TR-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. HiLyte Fluor™ 555-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. HiLyte Fluor™ 647-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. HiLyte Fluor™ 647-labeled)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
α-Actin smooth muscle
Anaspec
polyclonal
Rabbit
(liquid, PBS, BSA, azide)
-
Human, Mouse
immunogen = synthetic peptide corresp. to the N-terminus of human α-smooth muscle actin
alpha smooth muscle Actin specific
-
β-Actin (CT)
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, azide, purified)
-
Human, Mouse, Rat
immunogen = 16 aa peptide from near the C-terminal of human β-actin (Genbank AAH02409)
β-Actin
-
β-Actin (CT), Z-Fish
Anaspec
polyclonal
IgG
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified)
-
Zebra fish
immunogen = synthetic peptide derived neat the C-terminus of zebrafish β-actin (Genbank NP_571106)
β-Actin
-
β-Actin (NT)
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, azide, purified)
-
Human, Mouse, Rat
immunogen = 16 aa peptide from near the N-terminal of human β-actin (Genbank AAH02409)
β-Actin
-
β-Actin (NT), Z-Fish
Anaspec
polyclonal
IgG
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified)
-
Zebra fish
immunogen = synthetic peptide derived neat the N-terminus of zebrafish β-actin (Genbank NP_571106)
β-Actin
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid,, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T)
detects endogenous levels of total IRS1 protein
-
IRS1 phos Ser636/639
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 or serine 639 (M-Sp -P-K -Sp)
detects endogenous levels of IRS1 only when phosphorylated at Serine 636 or 639
-
IRS1 phos Tyr612
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, no preservative, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresponding to the epitope, D G Y M P, with a single phosphorylation site, Tyr612, from human IRS1
IRS1 when phosphorylated at Tyr 612
-
IRS1 (EP263Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA)
-
Human
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS-1
IRS1 (170kDa)
-
IRS1 phos Ser1101
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 1101 (H-S-Sp-E-T)
detects endogenous levels of IRS1 only when phosphorylated at serine 1101
-
IRS2 (EP903Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA)
-
Human
Immunogen = synthetic peptide corresp. to residues near the N-terminus of human IRS-2
IRS2 (170kDa)
-
IRS4 (EP907Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA)
-
Human
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS-4
IRS4 (Insulin receptor substrate 4) (133kDa)
-
IRS1 phos Ser312
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, no preservative, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide corresp. to the epitope A T Sp A, with a single phosphorylation site, Ser 312, from human IRS1
detects endogenous levels of IRS1 only when phosphorylated at serine 312
-
IRS1 phos Ser616
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide derived from a region of IRS 1 that contains serine 616
detects levels of IRS1 only when phosphorylated at serine 616
-
IRS2 phos Ser731
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide (Y K A Sp S P A E S S), corresp. to aa727-736 of human IRS2 at the phosphorylated site of Serine 731
IRS2 phosphorylated at ser731; not other phosphorylated sites or non-phosphorylated IRS 1 or IRS 2
-
IRS2 (EP976Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA)
-
Human
Immunogen = synthetic peptide corresp. to residues surrounding Ser1203 of human IRS2
IRS2 (170kDa)
-
IRS1 phos Tyr612
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Chicken, Human, Mouse, Rat
Immunogen = synthetic peptide derived from a region of IRS 1 that contains tyrosine 612
IRS1 when phosphorylated at Tyr 612; IRS 2 (85% homologous) has not been tested
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to the C-terminal 14 residues (Rat) conj. to KLH
IRS1 protein
-
IRS2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide derived from within residues 1300 to the C-terminus of mouse IRS2, conj. to KLH
IRS2 (170kDa)
-
IRS2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide derived from within residues 1050-1150 of mouse IRS2, conj. to KLH
IRS2 (170kDa)
-
IRS1 phos Ser307
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 307 (T-E-Sp-I-T)
detects levels of IRS1 only when phosphorylated at serine 307
-
IRS1 phos Ser312
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Pig, Human, Mouse, Rat
Immunogen = synthetic peptide (human) derived from a region of IRS 1 that contains serine 312
detects levels of IRS1 only when phosphorylated at serine 307
-
IRS1 phos Ser323
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized phosphopeptide derived from human IRS1 around the phosphoryation site of serine 323.
detects endogenous levels of IRS1 when phosphorylated at serine 323
-
IRS1 phos Ser616
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 612 (P-M-Sp-P-G).
detects endogenous levels of IRS1 only when phosphorylated at serine 616
-
IRS1 phos Ser636
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 (P-M-Sp-P-K).
detects IRS-1 only when phosphorylated at serine 636
-
IRS1 phos Ser639
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-Sp-V-S).
detects IRS-1 only when phosphorylated at serine 639; in rat and mouse the phosphorylation site is at serine 635
-
IRS1 phos Ser794
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-Sp-S-G).
detects IRS-1 only when phosphorylated at serine 794
-
IRS1 phos Tyr941
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human
Immunogen = synthetic peptide (human) derived from a region of IRS-1 that contains tyrosine 941
IRS1 when phosphorylated at Tyr 941
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human
Immunogen = synthetic peptide: I T A T S P A S, corresp. to aa308-315 of human IRS-1;in mouse and rat, the synthetic peptide is a portion of aa303-310
IRS1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636
detects endogenous levels of total IRS1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-Sp-O-S)
IRS-1
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 639 (P-K-Sp-V-S)
detects endogenous levels of total IRS1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 312 (A-T-Sp-O-S)
specific for IRS-1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-Sp-I-T)
specific for IRS-1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 or serine 639 (M-S-P-K-S)
detects endogenous levels of total IRS1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 323
IRS1 protein
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human
Immunogen = synthetic non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-Sp-S-G)
IRS1 protein
-
IRS2
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, azide, BSA, affinity purified)
-
Human
Immunogen = synthetic peptide corresp. to a region between residues 1288-1338 of human IRS2 (NP_003740.2)
IRS2
-
IRS1 phos Ser307
Abcam
polyclonal
IgG
Rabbit
(liquid, KPBS, BSA, azide, affinity purified)
-
Pig, Human, Mouse, Rat
Immunogen = synthetic phospho peptide: G L T R R S R T E Sp I T A T S P A S corresp. to aa298-316 of human IRS-1, conj. to KLH.
detects IRS-1 only when phosphorylated at serine 307 (180kDa)
-
IRS1 phos Ser522
Abcam
polyclonal
IgG
Sheep
(liquid, HEPES, NaCl, azide, affinity purified)
-
Chicken, Human, Mouse, Rat
Immunogen = synthetic peptide: C F R K R T H Sp A G T corresp. to aa521-530 of human IRS1, conjugated to KLH.
detects IRS-1 only when phosphorylated at serine 522
-
IRS1 phos Tyr1179
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide derived from a region of mouse IRS-1 that contains tyrosine 1173 (corresp. to tyrosine 1179 of the human seq.)
IRS1 when phosphorylated at Tyr 1179; IRS-3 (50% homologous) also may react, but has not been tested
-
IRS1 phos Tyr1229
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Chicken, Human
Immunogen = synthetic peptide derived from a region of human IRS-1 that contains tyrosine 1229
IRS1 when phosphorylated at Tyr 1229
-
IRS1 phos Tyr896
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide derived from a region of IRS 1 that contains tyrosine 896
IRS1 when phosphorylated at Tyr 896
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide mapping to the C- terminus of human IRS1
recognizes IRS1; no reaction to IRS2, IRS3 and IRS4
-
IRS1
Abcam
polyclonal
IgG
Sheep
(liquid, HEPES, NaCl, azide, affinity purified)
-
Chicken, Pig, Human, Mouse, Rat
Immunogen = synthetic peptide: C S I S F Q K Q P E D R Q corresp. to C-terminal aa1231-1242 of human IRS1, conj. to KLH
recognizes IRS1(200kDa)
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, Tris, azide, affinity purified, prediluted)
-
Chicken, Human, Mouse, Rat
Immunogen = synthetic peptide: S E D L S A Y A S I S F Q K Q P E D R Q, corresp. to amino acids 1223-1242 of human IRS1
recognizes IRS1
-
IRS2
Abcam
polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
-
Human, Hamster, Mouse, Rat
Immunogen = enzyme tagged protein, corresp. to C-terminal aa1122-1321 of mouse IRS2
IRS2 (170kDa); no cross with IRS-1
-
IRS4
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no preservative, protein G purified)
-
Human
Immunogen = recombinant fragment, corresp. to aa850-951 of human IRS4
IRS4 (insulin receptor substrate 4)
-
IRS4
Abcam
polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, glycerol, azide)
-
Human
Immunogen = synthetic peptide (human)
IRS4 (insulin receptor substrate 4)
-
IRS1 phos Ser307
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Mouse
Immunogen = synthetic peptide: E S I T A T Sp P A S M V G G K, corresp. to aa301-315 of human IRS-1.
detects IRS-1 only when phosphorylated at serine 307 (180kDa)
-
IRS1 phos Tyr1229
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human
Immunogen = phosphopeptide derived from a region of human IRS1 that contains tyrosine 1229
IRS1 when phosphorylated at Tyr 1229
-
IRS1 phos Tyr896 (EP260Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, glycerol, BSA, NaCl, Tris glycine)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS1, phosphorylated at tyrosine 896
IRS1 when phosphorylated at Tyr 896 (170kDa)
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, affinity purified)
-
Human, Mouse, Rat, African green monkey
Immunogen = synthetic peptide: E S I T A T S P A S M V G G K, corresp. to aa301-315 of human IRS-1
recognizes IRS1 (~170 kDa)
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, affinity purified)
-
Chicken, Human, Mouse, Rat
Immunogen = synthetic peptide: L H T D D G Y M P M S P G V, corresp. to aa602-615 of human IRS-1
recognizes IRS1 (~170 kDa)
-
IRS1
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, affinity purified)
-
Human, Mouse, Rat, Xenopus
Immunogen = synthetic peptide: D Y M P M S P K S V S A P Q Q I, corresp. to aa 631-646 of human IRS 1
recognizes IRS1 (~170 kDa)
-
IRS1 (8-36)
EMD Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (Y Y A S I N F Q K Q P E D R Q) corresp. to aa1221-1235 of rat insulin receptor substrate-1 (IRS-1), conjugated to KLH
recognizes the ~160-185 kDa IRS-1 protein
-
IRS2
EMD Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, undiluted serum, no preservative)
-
Human, Hamster, Mouse, Rat
Immunogen = recombinant protein containing aa1122-1321 of mouse IRS-2 fused to GST
recognizes the 180 kDa IRS-2 protein
-