Src phos Tyr527
Cell Signaling
polyclonal
IgG
Rabbit
(protein A and peptide affinity purifiedHEPES, saline BSA, glycerol)
-
Chicken, Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr527 of human Src
detects endogenous levels of Src only when phosphorylated at Tyr527; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites
-
Src phos Tyr416 (100F9)
Cell Signaling
monoclonal
IgG
Rabbit
(protein A and peptide affinity purifiedHEPES, saline BSA, glycerol)
-
Chicken, Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src
detects endogenous levels of Src only when phosphorylated at tyrosine 416; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites
-
Rac1
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide)
-
Human, Mouse
Immunogen = synthetic peptide consisting of the internal region of Rac1b that is not in the other Rac1 isoforms
recognizes Rac1b specifically; does not recognize other Rac1 isoforms
-
Rac1
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, PBS, saline, azide)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to aa 130-141 of human Rac1 (KEKKLT PITYPQ-C)
recognizes Rac1, does not cross react with Cdc42
-
Rac2
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Human, Mouse
Immunogen = fusion protein corresponding to residues 1-192 of human Rac2
21 kDa Rac2
-
Rac1 /Cdc42 phos Ser71
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, affinity purified)
-
Human
Immunogen = peptide corresp. to amino acid region encompassing the human, mouse, and rat phospho-Rac1/cdc42 (Ser71)
recognizes phosphorylated Rac1/cdc42 (Ser71)
-
Rac1 (23A8)
Upstate and Chemicon are Millipore
monoclonal
IgG2b
Mouse
(liquid, protein-G purified, Tris, azide)
-
Human, Mouse, Rat
Immunogen = recomb. protein containing full length human Rac
strong Rac1 preference with slight Rac2 cross-reactivity
-
Rac1 (23A8)
Upstate and Chemicon are Millipore
monoclonal
IgG2b
Mouse
(ascites, 50% slurry suspended in PBS, azide, agarose conj.)
-
Human, Mouse, Rat
Immunogen = recomb. protein containing full length human Rac1
strong Rac1 preference with slight Rac2 cross-reactivity
-
c-Src (17AT28)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse
immunogen = full-lengthrecombinant c-Src of human origin
detects c-Src
-
c-Src (4F1E8)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
-
Human
immunogen = a recombinantprotein corresp. to amino acids 10-193 of c-Src of human origin
detects c-Src
-
c-Src (5D10C4)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human
immunogen = a recombinantprotein corresp. to amino acids 10-193 of c-Src of human origin
detects c-Src
-
c-Src (N-16)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide, gelatin.Available as sc-19 PE, conjugate for FC, and sc-19 AC, conjugate for IP)
-
Human, Mouse, Rat
immunogen = apeptide mapping at the N-terminus of c-Src of human origin
detects c-Src
-
c-Src, C-term (B-12)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = c-Src of human origin (epitope between amino acids 506-537 at the C-terminus)
detects c-Src, Yes p62, Fyn p59, c-Fgrp55 and c-Src-2
-
c-Src, N-term (H-12)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = c-Src p60 of human origin (epitope between amino acids 1-30 at the N-terminus)
detects c-Src; specific for an epitope mapping between amino acids 1-30 at the N-terminus of c-Src p60 of human origin
-
c-Src (SRC 2)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = apeptide mapping at the C-terminus of Fyn p59 of human origin
detects c-Src, Yes p62, Fyn p59, c-Fgr p55 and c-Src-2
-
Cofilin (FL-166)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa1-166 representing full length of human cofilin
Cofilin 1 and Cofilin 2 and to a lesser extent, Destrin
-
Cofilin phos (hSer3)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Ser 3 of human cofilin
detection of Ser 3 phosphorylated Cofilin
-
Cofilin phos (mSer3)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = short amino acid seq. containing phosphorylated Ser 3 of mouse Cofilin
detection of Ser 3 phosphorylated Cofilin
-
Cofilin 1 (5)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
-
Cow, Pig, Human, Mouse, Rat
immunogen = full lenth human cofilin 1
Cofilin 1
-
Cofilin 2 (FG-7)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human
immunogen = recombinant human cofilin 2
Cofilin 2
-
Cofilin 2 (H-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin)
-
Mammal (various), Pig, Human, Mouse, Rat
immunogen = epitope mapping near the C-terminus of human Cofilin 2
Cofilin 2, muscle isoform; no cross-reaction with Cofilin 1, non-muscle isoform
-
Cofilin 1 (L-13)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mammal (various), Cow, Pig, Human, Mouse, Rat
immunogen = epitope mapping near the N-terminus of human Cofilin 1
Cofilin 1, non-muscle isoform; no cross-reaction with Cofilin 2, muscle isoform; may cross react with ADF
-
Cofilin (H-12)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Cow, Pig, Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus of human cofilin 1
Cofilin 1, non-muscle isoform; no cross-reaction with Cofilin 2, muscle isoform
-
Cofilin phos Ser3
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic phosphopeptide derived from a region of human cofilin that contains serine 3
cofilin phosphorylated at Ser3
-
Cofilin 2
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, TBS, azide, glycerol, protein A purified)
-
Chicken, Mouse, Rat
immunogen = synthetic peptide corresp. to aa136-152(C-Q V N G L D D I K D R S T L G E K) of human Cofilin 2 with N-terminal cysteine for KLH conj.
recognizes human Cofilin 2, Mr 21kD.
-
Cofilin 2 phos Ser3
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, TBS, azide, glycerol, protein A purified)
-
Chicken, Human, Mouse
immunogen = synthetic peptide corresponding to aa1-10 (M A [pS] G V T V N D E-C) of human phospho-Cofilin 2, with a C-terminal cysteine added for KLH conj.
recognizes phospho-Cofilin 2, Mr 21kD
-
Cofilin 2
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, TBS, BSA, azide, affinity purified)
-
Dog, Sheep, Pig, Mouse, Rat
immunogen = synthetic peptide corresp. to C- terminus of Cofilin 2 (muscle): peptide sequence (D I K D R S T L G E K)
recognizes human Cofilin 2, Mr 21kD.
-
ARC (CT)
Anaspec
polyclonal
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human
Immunogen = a synthetic peptide corresponding to the C-terminus of human ARC protein
detects ARC (Apoptosis Repressor with CARD)
-
ARC (NT)
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = a peptide corresp. to amino acids 2-18 of ARC of human origin
detects ARC (Apoptosis Repressor with CARD)
-
Cofilin 2 (6G9)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no preservative)
-
Human
immunogen = CFL2 (NP_068733, aa57-167 a.a) partial recombinant protein with GST tag.
Cofilin 2
-
Cofilin
Novus Biologicals
polyclonal
Rabbit
(liquid, PBS, no preservative)
-
Human, Mouse, Rat
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1
detects endogenous levels of Cofilin protein
-
Cofilin 2
Novus Biologicals
polyclonal
IgG
Goat
(liquid, TBS, BSA, azide)
-
Dog, Sheep, Pig, Human, Mouse, Rat
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1
recognize both reported isoforms (NP_068733 and NP_619579)
-
Cofilin phos Ser3
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
-
Cofilin phos ser3
-
SUMO-1 (C21A7)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide from the amino terminus of human SUMO-1
detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3
-
SUMO-1 (C9H1)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide from the amino terminus of human SUMO-1
detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3
-
SUMO-1 (2A12)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresp. to a sequence within human SUMO-1 that does not correspond to SUMO-2/3
detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3
-
SUMO-1
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresp. to a sequence within human SUMO-1 that does not correspond to SUMO-2/3
detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD)
-
SUMO-2/3 (81H8)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide from the amino terminus of human SUMO-3
recognizes endogenous levels of SUMO-2/3. It does not cross-react with recombinant SUMO-1
-
Dlx2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 100-200 of mouse DLX2, conj. to KLH
recognizes Dlx 2 (Distal-less (DII) family), on brain lysate
-
Dlx2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 200-300 of mouse DLX2, conj. to KLH
recognizes Dlx 2 (Distal-less (DII) family), on brain lysate (~40kDa); a number of larger bands are seen (~45, 50 and 60kDa) in some of the lysates run on WB (identity of these larger bands is unknown)
-
Dlx2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, sucrose, no preservative, protein A purified)
-
Human
immunogen = synthetic peptide: M T G V F D S L V A D M H S T Q I A A S S T Y H Q H Q Q P P S G G G A G P G G N S S S S S S L H K P, corresp. to N- terminal aa1-50 of human Dlx2
recognizes Dlx 2
-
Dlx2
Abcam
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, protein G purified)
-
Human, Mouse
immunogen = recombinant fragment, corresp. to aa1-110 of human Dlx2
recognizes Dlx2
-
Dlx2
Abcam
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, protein G purified)
-
Human, Mouse
immunogen = recombinant fragment, corresp. to aa1-110 of human Dlx2
recognizes Dlx2
-
Dlx2
Abcam
polyclonal
IgG
Rabbit
(lyophilized, PBS, ammonium sulfate, no preservative, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide: H Q H Q Q P P S G G G A G P G G, corresp. to aa24-39 of human Dlx2
recognizes Dlx2 (~34kDa)
-
Dlx2
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, thimerosal)
-
Human, Mouse
immunogen = 3 synthetic peptides (human) conjugated to KLH
recognizes Dlx2
-
Dlx2
Abcam
monoclonal
IgG;
Mouse
(liquid, PBS, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 100-200 of mouse Dlx2, conj. to KLH
recognizes Dlx2
-
Dlx2 (2C8)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, protein A purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-110) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (2H2)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, protein A purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-110) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (4B9)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, protein A purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-110) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (2E12)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-110) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (2B12)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-110) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (3H3)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, no preservative, purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-124) partial recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (3G6)
Novus Biologicals
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, no preservative, purified)
-
Human
immunogen = DLX2 (NP_004396, aa1-124) full length recombinant protein with GST tag
DLX2 - distal-less homeobox 2
-
Dlx2 (C-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping near the C-terminus of human Dlx-2
DLX-2
-
Dlx2 (O-23)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, sucrose, protein A purified)
-
Human
immunogen = synthetic human Dlx-2
DLX-2
-
Dlx2 (WQ6)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse
immunogen = recombinant human Dlx-2
DLX-2
-
Dlx2
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(lyophilized, protein A purified)
-
Human
immunogen = synthetic peptide corresp. to a region of human DLX2
DLX-2
-
Dlx2
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(lyophilized, protein A purified)
-
Dog, Mouse, Rat
immunogen = synthetic peptide corresp. to a region of human DLX2
DLX-2
-
Dlx2
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, no preservative, affinity purified)
-
Rodent (various)
immunogen = synthetic peptide corresp. to aa24-39 from human Dlx2
DLX-2
-
Stat1 phos Tyr701
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Bovine, Human, Mouse, Rat
immunogen = a synthetic phosphopeptide corresp. to residues surrounding Tyr701 of human Stat1
detects phosphorylated tyrosine 701 of p91 Stat1 and also the p84 splice variant; does not cross-react with the corresponding phospho-tyrosines of other Stat proteins
-
Stat1 phos Ser727
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Mouse, Rat
immunogen = a synthetic phosphopeptide corresp. to residues surrounding Ser727 of human Stat1
detects endogenous levels of Stat1α only when phosphorylated at Ser727; does not significantly cross-react with the corresponding phosphorylated residues of other Stat proteins
-
Stat1 phos Tyr701 (58D6)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding Tyr701 of human Stat1
detects phosphorylated tyrosine 701 of p91 Stat1 and also the p84 splice variant; does not cross-react with the corresp. phospho-tyrosines of other Stat proteins
-
Stat1, total
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Bovine, Human, Monkey, Mouse, Rat
immunogen = a synthetic phosphopeptide corresp. to the sequence of human Stat1
detects endogenous levels of total Stat1 protein; detects both Stat1alpha (91kDa) and Stat1beta (84 kDa) isoforms
-
Stat1, total (42H3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey
immunogen = a synthetic peptide corresponding to the sequence of human Stat1
detects endogenous levels of total Stat1 protein independent of phosphorylation
-
Stat1, total (9H2)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Bovine, Human
immunogen = a synthetic peptide
detects endogenous levels of total Stat1 protein independent of phosphorylation, however, it prefers the non-phosphorylated form of Stat1; does not significantly cross-react with the other Stat proteins
-