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340307 RESULTS

CX3CR1 (clone 22C7)

ALZANTIBODIES In initial testing on western blots, antibody 22C7 detected myc-tagged, but not untagged, CX3CR1 overexpressed in HEK293 cells. (It is possible that the myc tag stabilizes the CX3CR1 protein.) 22C7 detects myc-tagged human CX3CR1 overexpressed in HEK293 c

TMEM119 (clone 1G4)

ALZANTIBODIES In initial testing on western blots, antibody 1G4 reacted with human TMEM119 overexpressed in HEK293 cells. Antibody 1G4 recognizes human TMEM119 overexpressed in HEK293 cells. Western blot of lysates of HEK293 cells transfected with empty vector (mock),

TMEM119 (clone 23B6)

ALZANTIBODIES In initial testing on western blots, antibody 23B6 reacted with human TMEM119 overexpressed in HEK293 cells. Antibody 23B6 recognizes human TMEM119 overexpressed in HEK293 cells. Western blot of lysates of HEK293 cells transfected with empty vector (mock)

P2Y12R (clone 17A12)

ALZANTIBODIES Monoclonal antibody 17A12 reacts with the mouse P2Y12 receptor (P2Y12R). In initial testing, the antibody recognized its target on western blots of lysates from HEK293 cells that overexpress mouse P2Y12R. The antibody also stained microglia in mouse brain

P2Y12R (clone 1E5)

ALZANTIBODIES Monoclonal antibody 1E5 reacts with the mouse P2Y12 receptor (P2Y12R). In initial testing, the antibody recognized its target on western blots of lysates from HEK293 cells that overexpress mouse P2Y12R. The antibody also stained microglia in mouse brains.

CD33 (clone 17D5)

ALZANTIBODIES Monoclonal antibody 17D5 reacts with human CD33. In initial testing—on lysates of HEK293 cells that overexpressed myc-tagged human CD33 isoforms—it detected all three isoforms on Western blots and immunoprecipitated each isoform. The antibody was also sho

CD33 (clone 12C1)

ALZANTIBODIES Monoclonal antibody 12C1 reacts with human CD33. In initial testing—on lysates of HEK293 cells that overexpressed myc-tagged human CD33 isoforms—it detected all three isoforms on Western blots and immunoprecipitated each isoform. The antibody was also sho

TMEM119 (clone 5A4)

ALZANTIBODIES In initial testing on western blots, antibody 5A4 reacted with human TMEM119 overexpressed in HEK293 cells. Antibody 5A4 recognizes human TMEM119 overexpressed in HEK293 cells. Western blot of lysates of HEK293 cells transfected with empty vector (mock),

TYROBP/DAP12 (clone 9D10)

ALZANTIBODIES In initial testing, monoclonal antibody 9D10 recognized DAP12 endogenously expressed in the BV2 mouse microglial cell line, when used as a detection antibody for western blotting or a capture antibody for immunoprecipitation. The antibody also immunopreci

TYROBP/DAP12 (clone 14C2)

ALZANTIBODIES In initial testing, monoclonal antibody 14C2 recognized DAP12 endogenously expressed in the BV2 mouse microglial cell line, when used as a detection antibody for western blotting or a capture antibody for immunoprecipitation. The antibody also recognized

TYROBP/DAP12 (clone 4E12)

ALZANTIBODIES In initial testing, monoclonal antibody 4E12 recognized DAP12 endogenously expressed in the BV2 mouse microglial cell line, when used as a detection antibody for western blotting or a capture antibody for immunoprecipitation. The antibody also immunopreci

TYROBP/DAP12 (clone 1C8)

ALZANTIBODIES In initial testing, monoclonal antibody 1C8 recognized DAP12 endogenously expressed in the BV2 mouse microglial cell line, when used as a detection antibody for western blotting or a capture antibody for immunoprecipitation. The antibody also immunoprecip

Tau (AT8); Phospho Tau (Ser 202, Thr 205)

ALZANTIBODIES Hyperphosphorylated tau polymerizes into paired helical filaments, which aggregate to form neurofibrillary tangles, one of the defining lesions of Alzheimer’s disease. AT8, which recognizes paired helical filament (PHF) tau, is one of the most widely used

Tau (MC-1)

ALZANTIBODIES This monoclonal antibody was generated against paired helical filaments isolated from an Alzheimer’s brain. It recognizes a discontinuous epitope formed when tau folds in a way that brings together its N-terminal and third repeat regions. This tau conform

Tau (CP-13)

ALZANTIBODIES This monoclonal antibody, generated against paired helical filaments (PHFs) isolated from Alzheimer’s brains, recognizes tau phosphorylated at serine 202. CP-13 labels neurofibrillary tangles (NFTs), pretangles, neuropil threads, and dystrophic neurites i

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