AlzAntibodies
P2Y12R (clone 17A12)
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Immunogen: Peptide in C-terminus of murine P2Y12R
Clonality: Monoclonal
Isotype: IgG2c
Host: Rat
Reactivity: Mouse
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project P2YM, Clone 17A12.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass' laboratory |
Immunohistochemistry | Soyon Hong's laboratory |
Overview
Monoclonal antibody 17A12 reacts with the mouse P2Y12 receptor (P2Y12R). In initial testing, the antibody recognized its target on western blots of lysates from HEK293 cells that overexpress mouse P2Y12R. The antibody also stained microglia in mouse brains.
Antibody 17A12 recognizes murine P2Y12R overexpressed in HEK293 cells. Western blot of lysate of HEK cells transfected with empty vector (mock), mouse P2Y12R, myc-tagged mouse P2Y12R, or human P2Y12R, probed with hybridoma supernatant. 17A12 recognizes murine, but not human, P2Y12R. [Courtesy of Christian Haass' laboratory.]
Antibody 17A12 stains microglia in the mouse brain. Protein G-purified 17A12 stains cells with the morphology of microglia in paraformaldehyde-fixed sections through the mouse hippocampus. [Courtesy of Dimitra Sokolova, Soyon Hong's laboratory, UK DRI at UCL.]
Generation and epitope mapping
This antibody was generated in rats immunized with a peptide from the C-terminal region of murine P2Y12R, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
On western blots, 17A12 recognizes mouse, but not human, P2Y12R overexpressed in HEK293 cells.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
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