AlzAntibodies
CX3CR1 (clone 22C7)
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Immunogen: Peptide in C-terminus of human CX3CR1
Clonality: Monoclonal
Isotype: IgG2c
Host: Rat
Reactivity: Human
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project CX3S, Clone 22C7.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass' laboratory |
Overview
In initial testing on western blots, antibody 22C7 detected myc-tagged, but not untagged, CX3CR1 overexpressed in HEK293 cells. (It is possible that the myc tag stabilizes the CX3CR1 protein.)
22C7 detects myc-tagged human CX3CR1 overexpressed in HEK293 cells. Western blot of lysates from HEK293 cell transfected with empty vector (mock), human CX3CR1, or myc-tagged human CX3CR1, probed with 22C7 hybridoma supernatant. [Courtesy of Christian Haass' laboratory.]
Generation and epitope mapping
This antibody was generated in rats immunized with a peptide from the C-terminal region of human CX3CR1, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
Monoclonal antibody 22C7 is predicted to react with human CX3CR1.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
No Available Further Reading
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