Akt, immobilized (1G1)
Cell Signaling
monoclonal
IgG2a
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of human Akt
binds preferentially to Akt phosphorylated at Ser473; also binds to some non-phospho-Akt
-
Akt phos Thr308 duet
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 and Akt (pan) (C67E7) Rabbit mAb #4691)
-
Human, Monkey, Hamster, Mouse, Rat, Drosophila
-
assess protein activation status
-
Akt PhosphoPlus® Activation Kit
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb # 2965, Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb # 4060 and Akt (pan) (C67E7) Rabbit mAb # 4691)
-
Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Xenopus, Drosophila
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding either Ser473, Thr308 or from the C-terminal sequence of mouse Akt
detect endogenous levels of Akt only when phosphorylated at either Ser473 or Thr308; Also detects endogenous levels of total Akt1/2/3 independent of their phosphorylation state; no cross-reaction with related kinases.
-
Akt phos Ser473 (587F11)
Cell Signaling
monoclonal
IgG2b
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
reacts with endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Bovine, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of humanAkt (KLH-coupled)
reacts with endogenous Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
reacts with endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473
Cell Signaling
kit
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Chicken, Human, Hamster, Mouse, Rat
Akt pS473 (#9271) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt.Akt (#9272) = synthetic peptide derived from the C-terminal seq of mouse Akt.
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.Akt detects total Akt1, Akt2 and Akt3.
-
Akt phos Ser473
Cell Signaling
kit
Rabbit
(ready-to-use, Protein A and affinity purified)
-
Human
AktpSer473 (#9277) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.
-
Akt phospho Pathway Sampler Kit
Cell Signaling
kit
Rabbit
(multi antibody sampler kit; HEPES, saline, BSA, glycerol, azide)
-
Chicken, Human, Monkey, Mouse, Rat
various antibodies for phospho.Akt pathway: phospho Akt,pan Akt,phospho c-Raf,phospho GSK-3β,phospho PTEN,phospho PDK1
-
Akt phos Thr308 (244F9)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt.
reacts with Akt phosphor. at Thr308 only
-
Akt phos Thr308 (244F9)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Human
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt
reacts with Akt phosphorylated at Thr308 only
-
Akt phos Thr308 (L32A4)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
reacts with endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phopho-peptide corresp. to residues around Thr308 of mouse Akt
reacts with Akt phos Thr308 only. Does not detect Akt phosphorylated at other sites or related kinases
-
Akt phos Thr308
Cell Signaling
kit
Unknown Host
(capture ab = Akt rabbit #9272; detection ab = monoclonal Akt pThr308 #5103 PathScan®)
-
Human, Mouse
-
detects Akt when phosphorylated at Thr308
-
Akt phos Thr405
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phopho-peptide corresp. to residues around Thr451 of Akt2
reacts with endogenous levels of Akt1 only when phosphorylated at Thr450; also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues; not Akt phosphorylated at other sites
-
Akt phos Tyr326
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, protein A/affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Tyr326 of Akt (KLH coupled)
reacts with Akt only when phosphorylated at Tyr326; no cross-reaction with Akt phosphorylated at other sites
-
Akt Signal Stain®
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 and Akt (pan) (C67E7) Rabbit mAb #4691PTEN (D4.3) Rabbit mAb #9188)
-
Human, Mouse
immunogen = synthetic phospho-peptides corresp. to residues surrounding the phosphorylation site(s) of interest, and synthetic peptides derived from the C-terminal seq. of mouse Akt or from the C-terminal seq. of human PTEN
paraffin-embedded tissues or cells with antibodies directed against proteins involved in Akt signaling
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488)
-
Human
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647)
-
Human
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (587F11)
Cell Signaling
monoclonal
IgG2b
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase
-
Akt phos Ser473 (736E11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of mouse Akt (KLH-coupled)
detects Akt only when phosphorylated at Ser473 and Akt2 and Akt3 only when phosphorylated at equivalent sites
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Bovine, Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Thr308 (244F9H2)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
reacts with endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®488)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®647)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
ERK1 p44
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Protein A and affinity purified)
-
Mouse, Rat
Immunogen = synthetic peptide derived from mouse p44 MAP kinase (ERK1)
Recognizes endogenous levels of p44 MAP kinase (ERK1). Does not recognize p38 MAP kinase, p42 MAP kinase (ERK2) or SAPK/JNK.
-
ERK1/2 p44/42
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Protein A and affinity purified)
-
Zebra fish, Human, Hamster, Mouse, Rat
Immunogen = synthetic peptide derived from the sequence of rat p42 MAP kinase
Recognizes endogenous levels of total p44/42 MAP kinase (ERK1/2) protein. Does not recognize either JNK/SAPK or p38 MAP kinase.
-
ERK1/2 p44/42
Cell Signaling
kit
Mouse
(for IP crosslinked to agarose beads, cell lysis buffer, BSA, glycerol. Polyclonal rabbit for WB HEPES, BSA, NaCl, glycerol, purified)
-
Human, Mouse, Rat
Immunogen = IP synthetic phospho-Thr202/Tyr204 peptide of human p44 MAP kinase, KLH coupled. Immunogen = WB synthetic phospho-Ser383 of human Elk1, KLH coupled.
Measures p44/42 MAP kinase activity in the cell
-
ERK1/2 p44/42 MAPK (137F5)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide derived from the sequence of rat p44/42 MAP kinase, conj. to KLH
Recognizes endogenous levels of total p44/42 MAP kinase (Erk1/2). Does not cross-react with JNK/SAPK or p38 MAP kinase
-
ERK2 p42 total (3A7)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, BSA, HEPES, NaCl, glycerol, azide)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic peptide derived from the sequence of rat p42 MAP kinase coupled to KLH
Recognizes endogenous levels of total p42 MAP kinase (ERK2) protein. Does not cross-react with either JNK/SAPK or p38 MAP kinase
-
ERK2 p42 total (3A7)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, conjugated with Alexa Fluor® 488)
-
Human
Immunogen = synthetic peptide derived from the sequence of rat p42 MAP kinase, coupled to KLH
Recognizes endogenous levels of total p42 MAP kinase (ERK2) protein. Recognizes p44 MAP kinase (ERK1) in some cell types but with lower affinity. No cross reaction with JNK/SAPK or p38 MAP kinase
-
ERK2 p42 total (3A7)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, conjugated to Alexa Fluor ® 647)
-
Human, Mouse
Immunogen = synthetic peptide derived from the sequence of rat p42 MAP kinase
Recognizes endogenous levels of total p42 MAP kinase (ERK2) protein. Recognizes p44 MAP kinase (ERK1) in some cell types but with lower affinity. No cross reaction with JNK/SAPK or p38 MAP kinase.
-
ERK3
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Protein A and affinity purified)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide corresp. to residues surrounding Leu410 of human ERK3.
Recognizes endogenous levels of total ERK3 protein. No cross reaction with other ERK family members
-
ERK5
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, glycerol, Protein A and affinity purified)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide corresp. to human ERK5 coupled to KLH
Recognizes endogenous levels of total ERK5 protein. No cross-reaction with ERK1/2
-
ERK5 phos Thr218/Tyr220
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Protein A and affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr218/Tyr220 of human ERK5
Recognizes endogenous levels of ERK5 phosphorylated at Thr218/Tyr220. Cross reacts with ERK1/2. No cross reaction with phosphorylated p38 MAPK or SAPK/JNK
-
MAPK/CDK substrate phospho Thr
Cell Signaling
monoclonal
IgM
Mouse
(liquid, BSA, HEPES, NaCl, glycerol, azide, protein-A purified)
-
Non-specific
Immunogen = synthetic phospho-threonine- proline-containing peptides (KLH-coupled)
Reacts with phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context independent fashion
-
ERK2 p42
Cell Signaling
polyclonal
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Protein A and affinity purified)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide derived from human p42 MAP kinase (ERK2) coupled to KLH
Recognizes ERK2 (MAP kinase p42)Does not recognize p38 MAP kinase, p44 MAP kinase (ERK1) or SAPK/JNK
-
ERK1/2 p44/42 phos Thr202/Tyr204 (D13.14.4E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol)
-
Bovine, Dog, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, S. cerevisiae, Drosophila
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Detects endogenous levels of p42/44 MAP kinase (ERK1/2) only when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singularly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
-
ERK1/2 p44/42 phos Thr202/Tyr204 (D13.14.4E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, biotin conj.)
-
Bovine, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, S. cerevisiae, Drosophila
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Detects endogenous levels of p42/44 MAP kinase (ERK1/2) only when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singularly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
-
ERK1/2 p44/42 MAPK phos Thr202/Tyr204
Cell Signaling
kit
Unknown Host
-
Human, Mouse
-
Reacts with endogenous levels of p44/42 MAPK (Thr202/Tyr204) protein
-
ERK1/2 p44/42 MAPK phos Thr202/Tyr204 (E10)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, PBS, azide, conjugated to Alexa Fluor® 488)
-
Human, Mouse
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Recognizes endogenous levels of p44/42 MAP kinase (Erk1/2) dually phosphorylated at Thr202/Tyr204. Does not cross-react with the corresp. phosphorylated residues of either SAPK/JNK or p38 MAP kinase
-
ERK1/2 p44/42 MAPK phos Thr202/Tyr204 (E10)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, conj. to Alexa Fluor®647)
-
Human, Mouse
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44 MAP kinase, conjugated to KLH
Recognizes endogenous levels of p44/42 MAP kinase (Erk1/2) dually phosphorylated at Thr202/Tyr204. Does not cross-react with the corresp. phosphorylated residues of either SAPK/JNK or p38 MAP kinase
-
ERK1/2 p44/42 phos Thr202/Tyr204 (20G11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44/42 MAP kinase
Detects endogenous levels of p42/44 MAP kinase (ERK1/2) only when phosphorylated at Thr202/183 and Tyr204/185. Does not cross-react with phosphorylated residues of JNK/SAPK or p38 MAP kinase
-
ERK1/2 p44/42 MAPK phos Thr202/Tyr204
Cell Signaling
kit
Rabbit
(liquid, pre-diluted, ready-to-use)
-
Human, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44/42 MAP kinase, conjugated to KLH
Reacts with endogenous levels of p44/42 MAP kinase (Erk1/2) only when phosphorylated at Thr202/183 or Tyr204/185. Does not cross-react with the corresponding phosphorylated residues of SAPK/JNK or p38 MAP kinase
-
ERK1/2 p44/42 phos Thr202/Tyr204 (D13.14.4E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Alexa Fluor® 488 conj.)
-
Bovine, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, Drosophila
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Recognizes endogenous levels of p42/44 MAP kinase (ERK1/2) only when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singularly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
-
ERK1/2 p44/42 phos Thr202/Tyr204 (D13.14.4E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, BSA, HEPES, NaCl, glycerol, Alexa Fluor® 647 conj.)
-
Bovine, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, Drosophila
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Recognizes endogenous levels of p42/44 MAP kinase (ERK1/2) only when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singularly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
-
ERK1/2 p44/42 phos Thr202/Tyr204 (E10)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, PBS, Protein G affinity purified)
-
Zebra fish, Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Recognizes peptides derived from p44/42 MAPK phosphorylated at Thr202/Tyr204
-
Synaptophysin 1
Synaptic Systems
polyclonal
Rabbit
(lyophilized, antiserum)
-
Chicken, Frog, Zebra fish, Cow, Human, Hamster, Mouse, Rat
immunogen = synthetic peptide GPQGAPT SFSNQM (aa. 301-313 in human synaptophysin 1) coupled to KLH
Detects synaptophysin 1 with no cross-reactivity towards other synaptophysins
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(supernatant)
-
Bovine, Chicken, Human, Monkey, Mouse, Rat
-
recognizes SNAP 25
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Bovine, Porcine, Human, Hamster, Mouse, Rat, Xenopus
immunogen = synaptic vesicle-containing fractions IP from human brain homogenates using anti-human synaptophysin monoclonal antibodies
recognizes both the A and B isoforms of SNAP25, Mr ~27kDa
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
-
Human
-
recognizes SNAP 25
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Bovine, Porcine, Human, Hamster, Mouse, Rat, Xenopus
immunogen = synaptic vesicle-containing fractions immunoprecipitated
recognizes SNAP 25 both A and B isoforms
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(supernatant)
-
Porcine, Human, Hamster, Rat
immunogen = crude human synaptic immunoprecipitate
recognizes SNAP 25
-
SNAP 25
LifeSpan Biosciences, Inc.
polyclonal
Goat
(affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide of C-terminus of SNAP25 (D E A N Q R A T K M L G S G)
recognizes synaptosomal associated protein 25 (SNAP 25); recognise both reported isoforms NP_003072 and NP_570824
-
SNAP 25
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide
recognizes synaptosomal associated protein 25 (SNAP 25)
-
SNAP 25
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(unpurified serum)
-
Bovine, Mouse, Rat
immunogen = synthetic peptide from the C-terminal heptadeapeptide of mouse SNAP-25 corresp. to residues Cys+ 190-206: C R I D E A N Q R A T K M L G S G
recognizes synaptosomal associated protein 25 (SNAP 25); will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E; no crossreactivity with SNAP 23
-
SNAP 25
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(IgG fractionation)
-
Bovine, Zebra fish, Mouse, Rat
immunogen = synthetic peptide corresponding to aa 195-206
recognizes SNAP 25
-
SNAP 25
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(affinity purified)
-
Human
immunogen = synthetic peptide corresp. to aa195-206 from human SNAP25
recognizes synaptosomal associated protein 25 (SNAP 25)
-
Synaptophysin (7.2)
Li Starfish
monoclonal
IgG1
Mouse
(lyophilized, ascites)
-
Mammal (various), Avian (various), Amphibian (various)
immunogen = electro phoretically purified synaptophysin; reacts with cytoplasmic tail
recognizes Synaptophysin
-
Synaptophysin (7.2)
Li Starfish
monoclonal
IgG1
Mouse
(lyophilized, affinity purified)
-
Mammal (various), Avian (various), Amphibian (various)
immunogen = electro phoretically purified synaptophysin; reacts with cytoplasmic tail
recognizes Synaptophysin
-
Synaptophysin
Li Starfish
polyclonal
Rabbit
(lyophilized serum)
-
Frog, Cow, Human, Mouse, Rat
immunogen = synthetic peptide corr. to human aa. 301-313, coupled to KLH
recognizes Synaptophysin -neuronal synaptophysin is better recognised than neuroendocrine synaptophysin
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(ascites)
-
Porcine, Human, Hamster, Rat
immunogen = crude human synaptic immunoprecipitate
synaptosomal associated protein 25 (SNAP 25); recognizes fusion protein from COS cells but not from bacterial
-
SNAP 25
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein A purified)
-
Porcine, Human, Hamster, Rat
immunogen = crude human synaptic immunoprecipitate
synaptosomal associated protein 25 (SNAP 25); recognizes fusion protein from COS cells but not from bacterial
-
AP-2β (D-20)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin)
-
Mammal (various), Dog, Human, Mouse, Rat
immunogen = peptide mapping within an internal region of human AP-2β
AP-2β
-
AP-2β (H-20)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin)
-
Mammal (various), Dog, Human, Mouse, Rat
immunogen = peptide mapping within an internal region of human AP-2β
AP-2β
-
AP-2β (H-87)
Santa Cruz
polyclonal
IgG
Rabbit
(PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide corresp. to aa130-216 mapping within an internal region of human AP-2β
AP-2β
-
AP-2β (1G6/2)
Santa Cruz
monoclonal
IgG2b
Mouse
(PBS, azide, gelatin)
-
Human
immunogen = raised against truncated human AP-2 beta-protein prepared from bacteria
AP-2β
-
AP-2δ (J-18)
Santa Cruz
polyclonal
IgG
Rabbit
(PBS, azide, gelatin)
-
Dog, Zebra fish, Human, Mouse, Rat
immunogen = raised against human AP-2δ
AP-2δ
-
AP-2δ (Q-12)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope mapping within an internal region of human AP-2δ
AP-2δ; no cross-reaction with other AP-2 family members
-
AP-2δ (T-13)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human AP-2δ
AP-2δ; no cross-reaction with other AP-2 family members
-
AP-2ε (E-12)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping within an internal region of human AP-2ε
AP-2ε; no cross-reaction with other AP-2 family members
-
AP-2ε (N-12)
Santa Cruz
polyclonal
IgG
Goat
(PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the N-terminus of human AP-2ε
AP-2ε; no cross-reaction with other AP-2 family members
-
AP-2ε (T-25)
Santa Cruz
polyclonal
IgG
Rabbit
(PBS, azide, gelatin)
-
Human, Rat
immunogen = raised against human AP-2ε
AP-2ε
-
AP-2γ (6E4/4)
Santa Cruz
monoclonal
IgG1
Mouse
(PBS, azide, gelatin)
-
Human
immunogen = raised against bacterially produced AP-2 protein and specifically recognizes the C- terminus of AP-2γ
AP-2γ
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β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, azide)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
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β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 555 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 647 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 488 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, saline, KCl, sodium phosphatedibasic, BSA, glycerol)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the sequence of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β3-Tubulin (TU-20)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein
Recognizes endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1
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p53, paired 6
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (MEEPQSDPSVEP) corresponding to N-terminus of human p53
detects p53
-
p53, phos Ser9
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (SDPpSVEP) corresponding to human p53 at the phosphorylated site of Serine 9
detects p53 phosphoryl. at Serine 9
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p53, phos Ser15
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (PPLpSQET) corresponding to human p53 at the phosphorylated site of Serine 15
detects p53 phosphoryl. at Serine 15
-
p53, paired 9
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (MEEPQSDPSVEP) corresponding to N-terminus of human p53
detects p53
-
p53, paired 15
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (MEEPQSDPSVEP) corresponding to N-terminus of human p53
detects p53
-
p53, phos Ser18/23
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = a synthetic phosphopeptide (L-pS-QETF-pS-GL) corresponding to mouse p53 at the phosphorylated site of Serine 18 and 23
detects p53 phos at Serine 18 and 23
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p53, paired 23
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Monkey, Mouse
immunogen = a synthetic peptide (ETFSGLW) corresponding to amino acids surrounding Serine 23 of mouse p53
detects p53
-
p53, paired 18/23
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Monkey, Mouse
immunogen = a synthetic peptide (LSQETFSG) corresponding to amino acids surrounding Serine 18 and 23 of mouse p53
detects p53
-
p53 phos Ser18
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = a synthetic phosphopeptide (LPL-pS-QET) corresponding to mouse p53 at the phosphorylated site of Serine 18
detects p53 phosphor. at Serine 18
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p53 CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, MOPS, BSA, glycerol)
-
Zebra fish
immunogen = a synthetic peptide derived from the zebrafish p53 surrounding the C-terminal region (CT)
detects p53
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