Mutations: APP KM670/671NL (Swedish)
Modification: APP: Transgenic
Disease Relevance: Alzheimer's Disease, Cerebral Amyloid Angiopathy
Strain Name: N/A
Genetic Background: C57BL/6
Availability: Available through Material Transfer Management at Novartis
This widely-used and extensively characterized model was developed by colleagues at Novartis Pharma and the University of Basel. Mice have a 7-fold overexpression of human APP containing the Swedish mutation. The original publication (Sturchler-Pierrat et al., 1997) also described two additional models: APP14, which also contains the hAPP751 transgene bearing the Swedish mutation but utilized the human Thy1 promoter, and APP22, which was similar to the APP14 line except the hAPP751 transgene contained both the Swedish and London (V717I) mutations. These models are no longer available.
Transgene containing human APP (isoform 751) containing the Swedish (KM670/671NL) mutation under the murine Thy1 promoter.
Aβ deposits are first observed at 6 months of age. Congophilic plaques increase in size and number with age, occupying up to 25 percent of the neocortex and hippocampus in 24 month-old mice. Neocortical Aβ can been visualized with the PET tracer PiB at 12 months and beyond (Snellman et al., 2013). Plaques are surrounded by activated microglia, astrocytes, and dystrophic neurites containing hyperphosphorylated tau, although no neurofibrillary tangles are observed. Neuronal loss has been reported in the CA1 region of the hippocampus. Mice also develop CAA, and microhemorrages occur at later ages (Sturchler-Pierrat et al., 1997; Calhoun et al., 1998; Winkler et al., 2001).
Spatial memory defects in Morris Water maze at 3 months and progresses with age (Van dam et al., 2003; Kelly et al., 2003). Memory deficits in passive avoidance (shock) test were observed in 25 month-old mice, but not at younger ages (Kelly et al., 2003).
The CSF levels of Aβ and tau have also been examined. Aβ42 concentrations in CSF remain unchanged until 16 months, and then decline, with a 60 percent reduction by 6 months of age. Decreases in Aβ40 CSF concentrations were less prominent. CSF concentration of total tau is also elevated in 24-26 month-old APP23 animals (Maia et al., 2013).
For information regarding the availability of this model contact Material Transfer Management at Novartis.
When visualized, these models will distributed over a 18 month timeline demarcated at the following intervals: 1mo, 3mo, 6mo, 9mo, 12mo, 15mo, 18mo+.
- Synaptic Loss
- Changes in LTP/LTD
Congophillic, dense-core amyloid plaques first appear at 6 months, and increase in size and number with age. Amyloid plaques can occupy more than 25% of the neocortex and hippocampus in 24 month-old mice (Sturchler-Pierrat et al., 1997; Calhoun et al., 1998).
Dystrophic neurites containing hyperphopshorylated tau surounds Aβ plaques, but no neurofibrillary tangles are observed (Sturchler-Pierrat et al., 1997).
Neuronal loss (14-28 percent) has been reported in the CA1 region of the hippocampus in 14-18 month old mice (Calhoun et al., 1998).
Activated microglia in close proximity to dense amyloid plaques (Stalder et al., 1999). Upregulation of neuroinflammatory markers and activation of astrocytes and macrophages. Age-associated increase in components of the complement system, namely C1q and C3, at later ages (9 months and 18 months, respectively) (Reichwald et al., 2009).
Neocortical synapses were examined in mice as old as 24 months of age; no evidence of alterations in the number of synapses or levels of synaptophysin were observed (Boncristiano et al., 2005).
Changes in LTP/LTD
LTP in the hippocampus and prefrontal cortex is normal at all ages studied: 3, 6, 9, 12, 18 and 24 months (Roder at al., 2003).
Spatial memory defects in Morris Water maze at 3 months and progresses with age (Van dam et al., 2003; Kelly et al., 2003).
- Sturchler-Pierrat C, Abramowski D, Duke M, Wiederhold KH, Mistl C, Rothacher S, Ledermann B, Bürki K, Frey P, Paganetti PA, Waridel C, Calhoun ME, Jucker M, Probst A, Staufenbiel M, Sommer B. Two amyloid precursor protein transgenic mouse models with Alzheimer disease-like pathology. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13287-92. PubMed.
- Snellman A, López-Picón FR, Rokka J, Salmona M, Forloni G, Scheinin M, Solin O, Rinne JO, Haaparanta-Solin M. Longitudinal Amyloid Imaging in Mouse Brain with 11C-PIB: Comparison of APP23, Tg2576, and APPswe-PS1dE9 Mouse Models of Alzheimer Disease. J Nucl Med. 2013 Aug;54(8):1434-41. PubMed.
- Calhoun ME, Wiederhold KH, Abramowski D, Phinney AL, Probst A, Sturchler-Pierrat C, Staufenbiel M, Sommer B, Jucker M. Neuron loss in APP transgenic mice. Nature. 1998 Oct 22;395(6704):755-6. PubMed.
- Winkler DT, Bondolfi L, Herzig MC, Jann L, Calhoun ME, Wiederhold KH, Tolnay M, Staufenbiel M, Jucker M. Spontaneous hemorrhagic stroke in a mouse model of cerebral amyloid angiopathy. J Neurosci. 2001 Mar 1;21(5):1619-27. PubMed.
- Van Dam D, D'Hooge R, Staufenbiel M, Van Ginneken C, Van Meir F, De Deyn PP. Age-dependent cognitive decline in the APP23 model precedes amyloid deposition. Eur J Neurosci. 2003 Jan;17(2):388-96. PubMed.
- Kelly PH, Bondolfi L, Hunziker D, Schlecht HP, Carver K, Maguire E, Abramowski D, Wiederhold KH, Sturchler-Pierrat C, Jucker M, Bergmann R, Staufenbiel M, Sommer B. Progressive age-related impairment of cognitive behavior in APP23 transgenic mice. Neurobiol Aging. 2003 Mar-Apr;24(2):365-78. PubMed.
- Maia LF, Kaeser SA, Reichwald J, Hruscha M, Martus P, Staufenbiel M, Jucker M. Changes in amyloid-β and Tau in the cerebrospinal fluid of transgenic mice overexpressing amyloid precursor protein. Sci Transl Med. 2013 Jul 17;5(194):194re2. PubMed.
- Roder S, Danober L, Pozza MF, Lingenhoehl K, Wiederhold KH, Olpe HR. Electrophysiological studies on the hippocampus and prefrontal cortex assessing the effects of amyloidosis in amyloid precursor protein 23 transgenic mice. Neuroscience. 2003;120(3):705-20. PubMed.
- Reichwald J, Danner S, Wiederhold KH, Staufenbiel M. Expression of complement system components during aging and amyloid deposition in APP transgenic mice. J Neuroinflammation. 2009;6:35. PubMed.
- Boncristiano S, Calhoun ME, Howard V, Bondolfi L, Kaeser SA, Wiederhold KH, Staufenbiel M, Jucker M. Neocortical synaptic bouton number is maintained despite robust amyloid deposition in APP23 transgenic mice. Neurobiol Aging. 2005 May;26(5):607-13. PubMed.
- Stalder M, Phinney A, Probst A, Sommer B, Staufenbiel M, Jucker M. Association of microglia with amyloid plaques in brains of APP23 transgenic mice. Am J Pathol. 1999 Jun;154(6):1673-84. PubMed.