Research Models

APOE4 Targeted Replacement

Synonyms: APOE4 Humanized Knock-in

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Species: Mouse
Genes: APOE
Modification: APOE: Knock-In
Disease Relevance: Alzheimer's Disease
Strain Name: B6.129P2-Apoetm3(APOE*4)Mae N8
Genetic Background: 129 x C57BL/6, back-crossed to C57BL/6
Availability: Taconic: Stock# 1549-F or 1549-M

Summary

Gene targeting was used to replace the endogenous murine APOE gene with the human APOE4 allele. The mice are at increased risk of atherosclerosis compared with wild-type animals or mice expressing human APOE3. On a standard chow diet the mice have normal plasma levels of cholesterol and triglycerides, but altered relative levels of plasma lipoprotein particles. On a high fat diet they have elevated cholesterol, APOE, and APOB-48 compared with mice expressing human APOE3 (Knouff et al., 1999).

Modification Details

Targeted replacement of the endogenous murine APOE gene with the human APOE4 allele; targeting construct contained exons 2–4 of APOE4.

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References

Paper Citations

  1. . Apo E structure determines VLDL clearance and atherosclerosis risk in mice. J Clin Invest. 1999 Jun;103(11):1579-86. PubMed.

External Citations

  1. Taconic: Stock# 1549-F or 1549-M

Further Reading

Papers

  1. . Apolipoprotein E4 causes age- and Tau-dependent impairment of GABAergic interneurons, leading to learning and memory deficits in mice. J Neurosci. 2010 Oct 13;30(41):13707-17. PubMed.
  2. . Marked regional differences of brain human apolipoprotein E expression in targeted replacement mice. Neuroscience. 2004;124(4):725-33. PubMed.