Research Models
Selected Results
1 Models
Name | Other Names | Strain Name | Genetic Background | Gene | Mutation | Modification Info | Modification | Disease | Neuropathology | Behavior/Cognition | Other Phenotype | Availability | Primary Paper | Visualization | |
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Mouse Models (1)
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C57Bl/6J | Trem2 | TREM2 H157Y | CRISPR/Cas9 gene editing was used to introduce the H157Y mutation into the mouse Trem2 gene. | Trem2: Knock-In | Alzheimer's Disease | Microglial density and morphology did not differ between carriers of the H157Y variant and wild-type mice. | Trem2-H157Y knock-in did not differ from wild-type mice at 6 months of age in tests of anxiety, associative memory, and spatial working memory. | Enhanced hippocampal synaptic plasticity was observed in mice homozygous for the H157Y variant, compared with wild-type mice. | Available through Na Zhao (zhao.na@mayo.edu). | Qiao et al., 2023 | Yes |
1 Visualizations
AD-related Research Models
Phenotypes Examined
- Plaques
- Tangles
- Neuronal Loss
- Gliosis
- Synaptic Loss
- Changes in LTP/LTD
- Cognitive Impairment
When visualized, these phenotypes will distributed over a 18 month timeline demarcated at the following intervals: 3mo, 6mo, 9mo, 1yr, 15mo, 18mo+.
Trem2-H157Y knock-in
Observed
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Changes in LTP/LTD at 24
Enhanced paired-pulse facilitation and long-term potentiation at Schaffer collateral-CA1 synapses in 6-month-old mice homozygous for the H157Y Trem2 allele.
Absent
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Gliosis at
Microglial density and morphology did not differ between carriers of the H157Y variant and wild-type mice at 6 months of age.
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Synaptic Loss at
Levels of the presynaptic marker synaptophysin and the postsynaptic markers PSD95 and GLUR2 in the cortices of 6-month-old mice did not differ between carriers of the H157Y variant and wild-type mice.
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Cognitive Impairment at
The H157Y mutation did not affect the performances of 6-month-old mice on a battery of behavioral tests: the open-field test to assess anxiety, cued and contextual fear conditioning to measure associative memory, and the Y-maze test to measure spatial working memory.
No Data
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Plaques at
No data.
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Tangles at
No data.
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Neuronal Loss at
No data.
Genes | Mutations | Modification | Disease | Neuropathology | Behavior/Cognition |
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Trem2 | TREM2 H157Y | Trem2: Knock-In | Alzheimer's Disease | Microglial density and morphology did not differ between carriers of the H157Y variant and wild-type mice. |
Trem2-H157Y knock-in did not differ from wild-type mice at 6 months of age in tests of anxiety, associative memory, and spatial working memory. |