Antibody

TDP-43 phos Ser409/410 (1D3, 7A9)

Tools

Back to the Top

Clonality: monoclonal
Host: Rat
Isotype: IgG2a
Source: Manuela Neumann
Formulation: Cell culture supernatant
Reactivity:
Reacts in: Human, Mouse
Availability: Confirmed available Oct 2014

Immunogen/Epitope

immunogen = phosphopeptide pS409/410 (SMDSKS(p)S(p)GWG), corresponding to amino acids 404-413 of human TDP-43, coupled to BSA or ovalbumin.

Specificity

Detects C-terminal TDP-43 phosphorylated at serine 409 and 410. Does not react with the non-phosphorylated protein. By immunohistochemistry, labels a variety of TDP-43  inclusions, including neuronal cytoplasmic inclusions, dystrophic neurites, neuronal intranucelar inclusions, and glial cytoplasmic inclusions.

Comments / Questions

No Available Comments

Make a comment or submit a question

To make a comment you must login or register.

References

No Available References

Further Reading

Papers

  1. Igaz LM, Kwong LK, Chen-Plotkin A, Winton MJ, Unger TL, Xu Y, Neumann M, Trojanowski JQ, Lee VM. Expression of TDP-43 C-terminal Fragments in Vitro Recapitulates Pathological Features of TDP-43 Proteinopathies. J Biol Chem. 2009 Mar 27;284(13):8516-24. Epub 2009 Jan 21 PubMed.
  2. Dormann D, Capell A, Carlson AM, Shankaran SS, Rodde R, Neumann M, Kremmer E, Matsuwaki T, Yamanouchi K, Nishihara M, Haass C. Proteolytic processing of TAR DNA binding protein-43 by caspases produces C-terminal fragments with disease defining properties independent of progranulin. J Neurochem. 2009 Aug;110(3):1082-94. Epub 2009 Jun 9 PubMed.
  3. Lippa CF, Rosso AL, Stutzbach LD, Neumann M, Lee VM, Trojanowski JQ. Transactive response DNA-binding protein 43 burden in familial Alzheimer disease and Down syndrome. Arch Neurol. 2009 Dec;66(12):1483-8. PubMed.
  4. Mackenzie IR, Arzberger T, Kremmer E, Troost D, Lorenzl S, Mori K, Weng SM, Haass C, Kretzschmar HA, Edbauer D, Neumann M. Dipeptide repeat protein pathology in C9ORF72 mutation cases: clinico-pathological correlations. Acta Neuropathol. 2013 Dec;126(6):859-79. Epub 2013 Oct 6 PubMed.
  5. Igaz LM, Kwong LK, Lee EB, Chen-Plotkin A, Swanson E, Unger T, Malunda J, Xu Y, Winton MJ, Trojanowski JQ, Lee VM. Dysregulation of the ALS-associated gene TDP-43 leads to neuronal death and degeneration in mice. J Clin Invest. 2011 Feb;121(2):726-38. Epub 2011 Jan 4 PubMed.
  6. Mori K, Weng SM, Arzberger T, May S, Rentzsch K, Kremmer E, Schmid B, Kretzschmar HA, Cruts M, Van Broeckhoven C, Haass C, Edbauer D. The C9orf72 GGGGCC repeat is translated into aggregating dipeptide-repeat proteins in FTLD/ALS. Science. 2013 Mar 15;339(6125):1335-8. Epub 2013 Feb 7 PubMed.