Bad antibody sampler kit
Cell Signaling
kit
Unknown Host
(primary and secondaryantibodies for WB#9296; #5286; #9297; #9239; #7074 and #7076)
-
Human, Monkey, Mouse, Rat
-
#9296 endogenous levels of Bad when phosphorylated at Ser112#5286 Bad protein only when phosphorylated at Ser136#9297 detects transfected levels of Bad only when phosphorylated at Ser155#9239 detects endogenous levels of total Bad
-
Bax
DAKO
polyclonal
Rabbit
(antiserum, purified IgG fraction, Tris-HCl, azide, carrier protein)
-
Human
immunogen = synthetic peptide corresponding to amino acids 43-61 of the human Bax protein
rrecognizes Bax, 21 kDa
-
Bcl-2 (124)
DAKO
monoclonal
IgG1 κ
Mouse
(liquid, supernatant, Tris/HCl, azide)
-
Human
immunogen = synthetic peptide corr. to aa. 41-54 of bcl-2 protein
specific to Bcl-2, 25 kDa
-
Fas-CD95 (DX2)
DAKO
monoclonal
IgG1 κ
Mouse
(liquid, purified, BSA, azide, R-PE conj.)
-
Human
immunogen = CD95 transfected murine L cells
40-50 kDa Fas transfected cell lines but not with untransfected parental cells (FC)
-
Bad pSer99
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, PBS, BSA, azide, purified)
-
Human
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Ser99 of Bad
Bad only when phosphorylated at Ser99 (18kDa)
-
Bad pSer134
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, PBS, BSA, azide, purified)
-
Human
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Ser134 of Bad
Bad only when phosphorylated at Ser134 (18kDa)
-
Bad pSer75
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, PBS, BSA, azide, purified)
-
Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Ser75of Bad
Bad only when phosphorylated at Ser75 (18kDa)
-
Bad
Chemicon and Upstate are Millipore
polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
-
Human
immunogen = peptide of aa 87-103 of human Bad
Bad
-
Bad pSer112
Chemicon and Upstate are Millipore
polyclonal
IgG1
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Ser112 of human Bad
Bad only when phosphorylated at Ser112
-
Bad pSer128
Chemicon and Upstate are Millipore
polyclonal
IgG1
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Ser128 of mouse Bad
Bad only when phosphorylated at Ser128
-
Bad
MBL International
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, protein A/affinity purified)
-
Human, Mouse
immunogen = full-length aa 1-204 GST-mouse BAD
Bad; 30kDa in mouse and 25kDa in human
-
Bad
MBL International
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = purified murine Bad protein
detect the ~22 kDa Bad protein; no cross-reaction with related family members
-
Bad (pSer112)
MBL International
polyclonal
IgG1
Rabbit
(liquid, PBS, azide, BSA, glycerol, affinity purified)
-
Human, Mouse, Rat
-
detect Bad when phosphorylated at Ser112
-
Bad (BV48T)
MBL International
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, glycerol)
-
Human, Mouse, Rat
immunogen = purified murine Bad protein
detect the ~23 kDa Bad protein
-
Bad phos Ser155
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Serine 155 of human phos-BAD
detects endogenous levels of p-BAD protein when phosphorylated at Ser155
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues 110-160 of human BAD
recognizes an epitope within residues 110-160 of human BAD
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E)
detects endogenous levels of total BAD protein (specific to a.a. 155)
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)
detects endogenous levels of total BAD protein (specific to a.a. 112)
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, whole antiserum, PBS, azide)
-
Human
immunogen = full length recombinant protein corresponding to human Bad
Bad
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 136 (S-R-Sp-A-P)
detects endogenous levels of total BAD protein (specific to a.a. 136)
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues 120-168 of human BAD
recognizes an epitope within residues 120-168 of human BAD
-
Bad phos Ser136
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Serine 136 of human phos-BAD
recognizes an aa near S136
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Arginine 149 of human BAD
recognizes an amino acid near Arginine149
-
Bad phos Ser112
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Ser112 of human pBAD
recognizes an amino acid near Ser112
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, sera)
-
Human, Mouse, Rat
immunogen = synthetic peptide corrsp. to aa87-103 (G E E P S P F R G R S R S A P P N) of human Bad
Bad
-
Bad phos Ser112
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, thimerosal, glycerol, BSA, affinity purified)
-
Human
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser112 of mouse Bad
detects 23 kDa Bad only when phosphorylated at Ser112
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine136 (S-R-Sp-A-P)
recognizes an epitope around ser136 of BAD
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)
recognizes an epitope around ser112 of BAD
-
Bad pSer112
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)
recognizes BAD only when phosphorylated at Ser 112
-
Bad pSer155
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E)
recognizes BAD only when phosphorylated at Ser 155
-
Bad pSer75
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, azide,)
-
Human
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser75 of human BAD, (conjugated with KLH)
BAD - BCL2-antagonist of cell death [Ser75]
-
Bad pSer118
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Human
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser118 of human BAD, (conjugated with KLH)
BAD - BCL2-antagonist of cell death [Ser118]
-
Bad pSer134
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Human
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser134 of human BAD, (conjugated with KLH)
BAD - BCL2-antagonist of cell death [Ser134]
-
Bad pSer99
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Human
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser99 of human BAD, (conjugated with KLH)
BAD - BCL2-antagonist of cell death [Ser99]
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide)
-
Human, Mouse, Rat
immunogen = peptide corresp. to 15aa near the C-terminus of human Bad (GenBank accession no. Q92934)
Bad
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Threonine 118 of human BAD
Bad aa near Thr118
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E)
BAD
-
Bad pSer112
Novus Biologicals
kit
Unknown Host
(Antibody pair set content:1) Phospho-BAD Ser112 rabbit polyclonal antibody (20 ug)2) BAD mouse monoclonal antibody (40 ug))
-
Human
-
phospho-BAD (Ser112) & BAD dual recognition pair
-
Bad pSer75
Novus Biologicals
kit
IgG
Unknown Host
(Antibody pair set content:1) Phospho-BAD S75 rabbit polyclonal antibody (20 ug)2) BAD mouse monoclonal antibody (40 ug))
-
Human
-
phospho-BAD (Ser75) & BAD dual recognition pair
-
Bad pSer99
Novus Biologicals
kit
Unknown Host
(Antibody pair set content:1) Phospho-BAD S99 rabbit polyclonal antibody (20 ug)2) BAD mouse monoclonal antibody (40 ug))
-
Human
-
phospho-BAD (Ser99) & BAD dual recognition pair
-
Bad
Novus Biologicals
polyclonal
IgG
Chicken
(lyophilized, no preservative, affinity purified)
-
Human, Rat
immunogen = BAD fusion protein (2-168 aa)
BCL2-binding protein (BAD)
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human
immunogen = full length recombinant human BAD protein
Bad
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, thimerosal, affinity purified)
-
Human
immunogen = synthetic peptide surrounding aa115 of mouse Bad
Bad (22kDa); no cross-reaction with related family members
-
Bad phos Ser136
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine136 (S-R-Sp-A-P)
Bad when phosphorylated at Ser136
-
Bad (cleaved)
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Mouse, Rat
Immunogen: synthetic peptide corresp. to the residues surrounding Aspartate 71 of human cleaved-BAD
detects endogenous levels of Cleaved-BAD protein
-
Bad pSer155 (27AT937)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to seq. in Ser155 region of mouse Bad and residue Ser155 is phosphorylated
recognizes BAD only when phosphorylated at Ser 155
-
Bad pSer155 (27AT381)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to seq. in Ser155 region of mouse Bad and residue Ser155 is phosphorylated
recognizes BAD only when phosphorylated at Ser 155
-
Bad
Novus Biologicals
polyclonal
Rabbit
(liquid, no preservative, protein A purified)
-
Human
immunogen = BAD (NP_004313.1, aa1-168) full-length human protein
BAD - BCL2-antagonist of cell death
-
Bad pSer155 (27AT381)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, azide, affinity purified)
-
Mouse
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser155 of mouse Bad, (conjugated with KLH)
Bad - Bcl-associated death promoter (27AT381 ) [Ser155]
-
Bad pSer155
Novus Biologicals
kit
Unknown Host
(Antibody pair set content:1) Phospho-BAD Ser155 rabbit polyclonal antibody (20 ug)2) BAD mouse monoclonal antibody (40 ug))
-
Human
-
phospho-BAD (Ser155) & BAD dual recognition pair
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, KPBS, no preservative, affinity purified)
-
Human
-
Bad N-terminus
-
Bad
Novus Biologicals
polyclonal
Mouse
(liquid, antiserum, no preservative)
-
Human
immunogen = BAD (NP_004313.1, aa1-168) full-length human protein
BAD - BCL2-antagonist of cell death
-
Bad (3H8)
Novus Biologicals
monoclonal
IgG2a , λ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant BAD
BAD - BCL2-antagonist of cell death
-
Bad
Novus Biologicals
polyclonal
IgY
Chicken
(liquid, PBS)
-
Human, Rat
immunogen = recombinant fragment, corresp. to aa 2-168 of human Bad
Bad
-
Bad (Y208)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues in the N-term of human Bad
Bad; no cross-reaction with other Bcl-2 members
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, azide)
-
Human
immunogen = full length GST-human BAD fusion protein
BAD
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
-
Bad
-
Bad
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the residues surrounding Isoleucine 106 of human BAD
recognizes an aa near Isolucine 106
-
Bad (48)
BD Pharmingen
monoclonal
IgG2b
Mouse
(liquid, PBS, BSA, glycerol, azide, conj. FITC)
-
Human, Mouse, Rat
immunogen = peptide mapping from aa 39 to 198 of mouse Bad
Bad (23kDa)
-
Bad
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Mouse, Rat
immunogen = C-terminus of mouse Bad
Bad
-
Bad
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping near the N- terminus of mouse Bad
Bad
-