Species: Mouse
Genes: TREM2
Modification: TREM2: Knock-In
Disease Relevance: Alzheimer's Disease
Strain Name: C57BL/6J-Trem2^{em3(TREM2)Aduci}/J
Genetic Background: C57BL/6J
Availability: Register interest. The Jackson Laboratory Stock No. 038103.

Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) is a transmembrane receptor that modulates microglial activity and survival. A rare variant in TREM2, R47H, triples the risk of Alzheimer’s disease in heterozygous carriers.

This knock-in model carries a “humanized” TREM2 gene, in which the mouse genomic sequence from the start codon in exon 1 to the stop codon in exon 5 was replaced with the corresponding human sequence. These mice serve as controls for the hTREM2-R47H_KI line, which carries a humanized TREM2 gene with the R47H variant.

The levels of expression of the humanized allele and wild-type mouse allele are similar in heterozygous mice, as assessed by RNA-Seq of hippocampal tissue from 2-month-old animals.

Both heterozygous and homozygous mice are viable and fertile.

Modification Details

Mouse genomic DNA between the start codon in exon 1 and the stop codon in exon 5 was replaced by the corresponding human genomic DNA sequence.

Phenotype Characterization

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References

Research Models Citations

1. hTREM2-R47H_KI

External Citations

1. The Jackson Laboratory Stock No. 038103

Further Reading

No Available Further Reading