Research Models
APOE4 Knock-In (JAX)
Synonyms: APOE4 KI, APOE*4 KI
Quick Links
Species: Mouse
Genes: APOE
Modification: APOE: Knock-In
Disease Relevance: Alzheimer's Disease
Strain Name: B6(SJL)-ApoEtm1.1(APOE*4)Adiuj/J
Genetic Background: C57BL/6J
Availability: The Jackson Laboratory, Stock # 027894; Live
The APOE4 knock-in model is one line in a set of three APOE knock-in lines created by The Jackson Laboratory. APOE2 and APOE3 mice are also available. A detailed description of these lines can be found on the APOE3 knock-in page.
Modification Details
The Apoe gene in C57BL/6J mouse embryonic stem cells was humanized through homologous recombination using a targeting vector containing 1.5 kb of human APOE gene sequence (the APOE4 isoform) including exons 2, 3, 4, and a portion of the 3' UTR sequence (this vector also contained a FRT-flanked Neo cassette to select for stem cells that had successfully integrated the vector). Mice were generated by injection of the genetically modified stem cells into donor embryos. Chimeric animals were bred to C57BL/6J mice, and progeny were crossed to FLP recombinase-expressing mice to remove the Neo cassette. The resulting Neo-negative mice were then backcrossed to C57BL/6J animals to remove the FLP recombinase transgene.
Related Strains
Trem2 R47H KI x APOE4. This double-mutant line (The Jackson Lab:Stock# 028709) was generated by crossing the line carrying a humanized APOE4 gene (Jackson Lab Stock #027894) to a line carrying an R47H point mutation knocked into the mouse Trem2 gene (Jackson Lab Stock #027918). The APOE4/Trem2*R47H line, also known as "LOAD1," serves as a standard background, or “platform strain,” for the introduction of additional alleles associated with late-onset AD in the MODEL-AD project.
Last Updated: 28 Feb 2025
References
Research Models Citations
Other Citations
External Citations
Further Reading
No Available Further Reading
COMMENTS / QUESTIONS
No Available Comments
Make a comment or submit a question
To make a comment you must login or register.