PI: Takaomi Saido
Affiliation: RIKEN Brain Science Institute
Submitted by: Nobuhisa Iwata
Posted Date: 24 Feb 2009

Brief Description

This protocol is a method to extract Aβ from brain tissues for application to Aβ ELISA.

References

1. Saido TC, Iwata N. Metabolism of amyloid beta peptide and pathogenesis of Alzheimer's disease. Towards presymptomatic diagnosis, prevention and therapy. Neurosci Res. 2006 Apr;54(4):235-53. Epub 2006 Feb 2 PubMed.
2. Huang SM, Mouri A, Kokubo H, Nakajima R, Suemoto T, Higuchi M, Staufenbiel M, Noda Y, Yamaguchi H, Nabeshima T, Saido TC, Iwata N. Neprilysin-sensitive synapse-associated amyloid-beta peptide oligomers impair neuronal plasticity and cognitive function. J Biol Chem. 2006 Jun 30;281(26):17941-51. Epub 2006 Apr 24 PubMed.
3. Saito T, Iwata N, Tsubuki S, Takaki Y, Takano J, Huang SM, Suemoto T, Higuchi M, Saido TC. Somatostatin regulates brain amyloid beta peptide Abeta42 through modulation of proteolytic degradation. Nat Med. 2005 Apr;11(4):434-9. Epub 2005 Mar 20 PubMed.
4. Iwata N, Mizukami H, Shirotani K, Takaki Y, Muramatsu S, Lu B, Gerard NP, Gerard C, Ozawa K, Saido TC. Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-beta peptide in mouse brain. J Neurosci. 2004 Jan 28;24(4):991-8. PubMed.
5. Iwata N, Tsubuki S, Takaki Y, Shirotani K, Lu B, Gerard NP, Gerard C, Hama E, Lee HJ, Saido TC. Metabolic regulation of brain Abeta by neprilysin. Science. 2001 May 25;292(5521):1550-2. PubMed.

Keywords

Aβ, ELISA, brain

Further Reading

No Available Further Reading