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Korin B, Ben-Shaanan TL, Schiller M, Dubovik T, Azulay-Debby H, Boshnak NT, Koren T, Rolls A. High-dimensional, single-cell characterization of the brain’s immune compartment. Nat Neuro 24 July 2017
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Stanford University
This is one of the first characterizations of brain myeloid compartment cells by CYTOF—which allows for multidimensional analysis on multiple protein markers on a single cell.
The value of this manuscript as a resource is not only as a standalone paper, but also as the baseline against which to compare future CYTOF-based analyses of brain pathology, AD included.
A paramount challenge of most cytometry is that it is only as valid as the antibodies used to detect the proteins of interest—and there are so many bad antibodies out there that love to stick to brain myeloid cells! I was delighted to see the thoughtful consideration of this limitation by the authors’ inclusion of corresponding RNAseq data of their major findings.
I can't wait to dig into their data set even more! Their validation of CD44 as a marker of all "infiltrating" or "surveying" immune cells in the CNS is exciting—especially in considering what this means ... how does CD44 contribute to brain immune surveillance at baseline and in disease?
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