Kass B, Schemmert S, Zafiu C, Pils M, Bannach O, Kutzsche J, Bujnicki T, Willbold D. Aβ oligomer concentration in mouse and human brain and its drug-induced reduction ex vivo. Cell Rep Med. 2022 May 17;3(5):100630. PubMed.

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  1. Reading this contribution, one may obtain the impression that there are "cheap" assays available that quantitate oligomers with the required sensitivity. As a comment, I just would like to add that I am unaware of another assay (besides sFIDA) that is reliably and robustly able to quantify protein aggregates, regardless of their particular conformation, down to the low femtomolar range. Recent examples for the usefulness and the reliability of sFIDA are the publications by Blömeke et al., 2022, and Schaffrath et al., 2023.

    References:

    Blömeke L, Pils M, Kraemer-Schulien V, Dybala A, Schaffrath A, Kulawik A, Rehn F, Cousin A, Nischwitz V, Willbold J, Zack R, Tropea TF, Bujnicki T, Tamgüney G, Weintraub D, Irwin D, Grossman M, Wolk DA, Trojanowski JQ, Bannach O, Chen-Plotkin A, Willbold D. Quantitative detection of α-Synuclein and Tau oligomers and other aggregates by digital single particle counting. NPJ Parkinsons Dis. 2022 Jun 2;8(1):68. PubMed.

    Schaffrath A, Schleyken S, Seger A, Jergas H, Özdüzenciler P, Pils M, Blömeke L, Cousin A, Willbold J, Bujnicki T, Bannach O, Fink GR, Willbold D, Sommerauer M, Barbe MT, Tamgüney G. Patients with isolated REM-sleep behavior disorder have elevated levels of alpha-synuclein aggregates in stool. NPJ Parkinsons Dis. 2023 Feb 2;9(1):14. PubMed.

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