Antibody

FLAG (M2)

Tools

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Clonality: monoclonal
Host: Mouse
Isotype: IgG1
Source: Sigma Aldrich
Cat# F4049
Reference:
(for IF, but unspecified conjugation,
Crippa, 2010 )
Formulation: purified Ig, buffered aqueous solution, PBS, BSA, azide, FITC
Reactivity:
Reacts in: Various species, Mouse, Rat

Specificity

Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host

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References

Paper Citations

  1. Crippa V, Sau D, Rusmini P, Boncoraglio A, Onesto E, Bolzoni E, Galbiati M, Fontana E, Marino M, Carra S, Bendotti C, De Biasi S, Poletti A. The small heat shock protein B8 (HspB8) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). Hum Mol Genet. 2010 Sep 1;19(17):3440-56. PubMed.

Further Reading

No Available Further Reading