AlzAntibodies
ApoE (clone 5E8)
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Immunogen: Recombinant human ApoE
Clonality: Monoclonal
Isotype: IgG2a
Host: Rat
Reactivity: Human
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project APOE, Clone 5E8.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass' laboratory |
Immunofluorescence | Beth Stevens' laboratory |
Overview
In initial testing on western blots, antibody 5E8 reacted with all three isoforms of human ApoE overexpressed in CHO cells. The antibody also stained THP1 cells, a human monocyte cell line derived from a patient with acute monocytic leukemia.
Antibody 5E8 reacts with all three isoforms of human ApoE overexpressed in CHO cells. Western blots of conditioned media from CHO cells transfected with one of the three major alleles of human APOE, probed with 5E8 hybridoma supernatant or a commercially available anti-ApoE antibody (SC-E8; Santa Cruz #sc-393302). -, medium from untransfected CHO cells. [Courtesy of Christian Haass' laboratory.]
5E8 immunofluorescence in THP1 cells. THP1 cells immunostained with antibody 5E8, followed by a fluorescently-conjugated secondary antibody (red); nuclei counterstained with DAPI (blue). [Courtesy of Trevor Atkeson, Beth Stevens' laboratory.]
Generation and epitope mapping
This antibody was generated in rats immunized with recombinant human ApoE, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
5E8 recognizes human ApoE.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
No Available Further Reading
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