AlzAntibodies
ApoE (clone 8D4)
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Immunogen: Recombinant human ApoE
Clonality: Monoclonal
Isotype: IgG2a
Host: Rat
Reactivity: Human
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project APOE, Clone 8D4.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass' laboratory |
Overview
In initial testing on western blots, antibody 8D4 reacted with all three isoforms of human ApoE overexpressed in CHO cells.
Antibody 8D4 reacts with all three isoforms of human ApoE overexpressed in CHO cells. Western blots of conditioned media from CHO cells transfected with one of the three major alleles of human APOE, probed with 8D4 hybridoma supernatant or a commercially available anti-ApoE antibody (SC-E8; Santa Cruz #sc-393302). -, medium from untransfected CHO cells. [Courtesy of Christian Haass' laboratory.]
Generation and epitope mapping
This antibody was generated in rats immunized with recombinant human ApoE, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
8D4 recognizes human ApoE.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
No Available Further Reading
Comments
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