Immunogen: Peptide in N-terminal region of mouse ApoE
Clonality: Monoclonal
Isotype: IgG2b
Host: Mouse
Reactivity: Mouse

Overview

In initial testing on western blots, antibody 26C11 reacted with murine ApoE endogenously expressed by the BV2 microglial cell line. The antibody also stained ApoE associated with amyloid plaques in the brains of APPPS1 mice.

Antibody 26C11 recognizes mouse ApoE endogenously expressed by BV2 cells. Western blots of conditioned media from BV2 cultures, probed with 26C11 hybridoma supernatant and two commercially available anti-ApoE antibodies, Abcam ab183596 and Creative Biolabs HJ6.3. Arrows show mouse ApoE. [Courtesy of Christian Haass' laboratory.]

Antibody 26C11 stains plaque-associated ApoE in the brains of APPPS1 mice. Left, 26C11-immunoreactive ApoE (green) is located within amyloid plaques (magenta, stained with polyclonal rabbit-anti-Aβ antibody 3552). Right, same field showing ApoE (green) and the microglial marker Iba1 (red). [Courtesy of Laura Sebastian Monasor, Sabina Tahirovic's laboratory, DZNE Munich.]

Generation and epitope mapping

This antibody was generated in mice immunized with a peptide from the N-terminal region of mouse ApoE, coupled to ovalbumin. Epitope mapping has not been reported.

Specificity

26C11 recognizes mouse ApoE.

Validation

No data.

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References

Research Models Citations

1. APPPS1

Further Reading

No Available Further Reading