AlzAntibodies

ABI3 (clone 3C4)

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Immunogen: Peptide in C-terminus of murine ABI3
Clonality: Monoclonal
Isotype: IgG2a
Host: Rat
Reactivity: Mouse

RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project ABIM, Clone 3C4.

Application Reference(s)

Western blot

Christian Haass' laboratory

Overview

Monoclonal antibody 3C4 reacts with mouse ABI3. In initial testing, the antibody was shown to work as a detection reagent for western blotting.

 

Antibody 3C4 recognizes murine ABI3 overexpressed in HEK293 cells. Western blot of lysate of HEK cells transfected with empty vector (mock), human ABI3, or murine Abi3, probed with hybridoma supernatant. 3C4 recognizes murine, but not human, ABI3. [Courtesy of Christian Haass' laboratory.]

Generation and epitope mapping

This antibody was generated in rats immunized with a peptide from the C-terminal region of murine ABI3 coupled to ovalbumin. Epitope mapping has not been reported.

Specificity

On Western blots, 3C4 recognizes murine, but not human, ABI3 overexpressed in HEK293 cells.

Validation

No data.

Last Updated: 15 Dec 2022

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