Antibody

Amyloid-β protofibrils

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Clonality: monoclonal
Host: Unknown Host
Isotype: IgG2a
Source: mAb158
(Hillevi Englund, Department of Public Health/Molecular Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden)
References:
Lord, 2009
Englund, 2007.
Recently, Anna Lord reported that the mAb158 sandwich ELISA detects synthetic protofibrils, as well as protofibrils in biological samples from a variety of mouse models
Formulation: supernatant, protein-G purified
Reactivity:
Reacts in: Human

Immunogen/Epitope

immunogen = 30 μg Aβ1–42 Arc protofibrils

Specificity

beta amyloid protofibrils; does not bind to other amyloids or to amyloid precursor protein (APP). The conformation selectivity of mAb158 is exclusive for amyloid-β (Aβ) as it did not display any binding to other amyloid fibrils; mAb158 did not immuno precipitate α-APPs from cell media or full-length APP from mouse brain homogenate; binds to Aβ fibrils in addition to oligomeric Aβ; has some cross-reactivity with monomeric Aβ

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References

News Citations

  1. San Diego: Oligomers Live Up to Bad Reputation, Part 2

Paper Citations

  1. Lord A, Gumucio A, Englund H, Sehlin D, Sundquist VS, Söderberg L, Möller C, Gellerfors P, Lannfelt L, Pettersson FE, Nilsson LN. An amyloid-beta protofibril-selective antibody prevents amyloid formation in a mouse model of Alzheimer's disease. Neurobiol Dis. 2009 Dec;36(3):425-34. PubMed.
  2. Englund H, Sehlin D, Johansson AS, Nilsson LN, Gellerfors P, Paulie S, Lannfelt L, Pettersson FE. Sensitive ELISA detection of amyloid-beta protofibrils in biological samples. J Neurochem. 2007 Oct;103(1):334-45. PubMed.

Further Reading

No Available Further Reading