AlzAntibodies

CX3CR1 (clone 27C3)

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Epitope: N/A
Immunogen: Peptide in C-terminus of human CX3CR1
Clonality: Monoclonal
Isotype: IgG3
Host: Mouse
Reactivity: Human

RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project CX3S, Clone 27C3.

Application Reference(s)

Western blot

Christian Haass' laboratory

Overview

In initial testing on western blots, antibody 27C3 detected myc-tagged, but not untagged, CX3CR1 overexpressed in HEK293 cells. (It is possible that the myc tag stabilizes the CX3CR1 protein.)

 

27C3 detects myc-tagged human CX3CR1 overexpressed in HEK293 cells. Western blot of lysates from HEK293 cell transfected with empty vector (mock), human CX3CR1, or myc-tagged human CX3CR1, probed with 27C3 hybridoma supernatant. [Courtesy of Christian Haass' laboratory.]

Generation and epitope mapping

This antibody was generated in mice immunized with a peptide from the C-terminal region of human CX3CR1, coupled to ovalbumin. Epitope mapping has not been reported.

Specificity

Monoclonal antibody 27C3 is predicted to react with human CX3CR1.

Validation

No data.

Last Updated: 15 Dec 2022

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References

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Further Reading

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