Immunogen: Peptide in C-terminus of isoform-2 of murine CD33
Clonality: Monoclonal
Isotype: IgG2c
Host: Rat
Reactivity: Mouse

Overview

Monoclonal antibody 8C1 reacts with mouse CD33m. In initial testing, the antibody was shown to work as a detection reagent in western blotting and immunocytochemical applications and as a capture reagent for immunoprecipitation.

Monoclonal antibody 8C1 detects mouse CD33m overexpressed in HEK293 cells. A) Western blot of lysates of HEK cells transfected with myc-tagged mouse CD33m or empty vector (mock), probed with 8C1. B) Lysates immunoprecipitated with 8C1; blot probed with anti-myc antibody 9E10. *, non-specific bands. [Courtesy of Christian Haass' laboratory.]

Monoclonal antibody 8C1 recognizes mouse CD33m overexpressed in HEK cells. HEK cells transfected with human CD33 (isoform CD33-3 or CD33m) or mouse (transcript 2, also called CD33m) isoforms were fixed, permeabilized, then incubated with 8C1 followed by a fluorescently conjugated secondary antibody. Cultures were counterstained with the nuclear stain DAPI (blue). Note 8C1 immunoreactivity (green puncta) in the  mouse CD33m-transfected cells. As expected, this antibody did not recognize human CD33 isoforms. [Courtesy of Christian Haass' laboratory.]

Generation and epitope mapping

This antibody was generated in rats immunized with a peptide in the C-terminal region of isoform-2 (CD33m) of murine CD33, coupled to ovalbumin. Epitope mapping has not been reported.

Specificity

Based on the amino acid sequence of the peptide immunogen, clone 8C1 is expected to react with the short (CD33m)—but not long (CD33M)—isoform of the mouse protein. The antibody does not react with human CD33.

Validation

No data.

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References

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Further Reading

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