AlzAntibodies
CD33 (clone 3F5)
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Immunogen: Peptide in C-terminus of isoform-2 of murine CD33
Clonality: Monoclonal
Isotype: IgG2c
Host: Rat
Reactivity: Mouse
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project CD33B, Clone 3F5.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass’ laboratory |
Immunoprecipitation | Christian Haass’ laboratory |
Overview
Monoclonal antibody 3F5 reacts with mouse CD33m. In initial testing, the antibody was shown to work as a detection reagent in western blotting and as a capture reagent for immunoprecipitation.
Monoclonal antibody 3F5 recognizes mouse CD33m overexpressed in HEK cells. A) Western blot of lysates of HEK293 cells transfected with myc-tagged mouse CD33m or empty vector (mock), probed with 3F5. B) Lysates immunoprecipitated with 3F5; blot probed with anti-myc antibody 9E10. *, non-specific bands. [Courtesy of Christian Haass' laboratory.]
Generation and epitope mapping
This antibody was generated in rats immunized with a peptide in the C-terminal region of isoform-2 (CD33m) of murine CD33, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
Based on the amino acid sequence of the peptide immunogen, clone 3F5 is expected to react with the short (CD33m)—but not long (CD33M)—isoform of the mouse protein. The antibody is not expected to react with human CD33.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
No Available Further Reading
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