AlzAntibodies
CD33 (clone 2C11)
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Immunogen: Peptide in C-terminus of human CD33
Clonality: Monoclonal
Isotype: IgG2b
Host: Rat
Reactivity: Human
RRID: N/A
Availability: Available through the Monoclonal Antibody Core Facility at Helmholtz Center Munich. Please request as Project CD33S, Clone 2C11.
| Application | Reference(s) |
|---|---|
Western blot | Christian Haass’ laboratory |
Immunoprecipitation | Christian Haass’ laboratory |
Overview
Monoclonal antibody 2C11 reacts with human CD33. In initial testing—on lysates of HEK293 cells that overexpressed myc-tagged human CD33 isoforms—it detected all three isoforms on Western blots and immunoprecipitated each isoform.
Monoclonal antibody 2C11 recognizes human CD33 overexpressed in HEK cells. A) Western blots of lysates of HEK293 cells transfected with myc-tagged human CD33 isoforms or empty vector (mock); blot probed with 2C11. Note that 2C11 recognizes all three isoforms of human CD33. B) Left blot, lysates immunoprecipitated with 2C11. Right blot, cell lysates (input for immunoprecipitation). Blots probed with anti-myc antibody 9E10. All three CD33 isoforms are captured by 2C11. *, non-specific bands. [Courtesy of Christian Haass' laboratory.]
Generation and epitope mapping
This antibody was generated in rats immunized with a peptide in the C-terminal region of human CD33 that is present in all three isoforms, coupled to ovalbumin. Epitope mapping has not been reported.
Specificity
Clone 2C11 reacts with all three isoforms of the human protein. The antibody is not expected to react with mouse CD33.
Validation
No data.
Last Updated: 15 Dec 2022
References
No Available References
Further Reading
No Available Further Reading
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