Immunogen: Peptide in C-terminus of human CD33
Clonality: Monoclonal
Isotype: IgG2c
Host: Rat
Reactivity: Human

Overview

Monoclonal antibody 10B2 reacts with human CD33. In initial testing on lysates of HEK293 cells that overexpressed myc-tagged human CD33 isoforms, the antibody immunoprecipitated all three human isoforms, but preferentially captured CD33m. Similarly, the antibody preferentially detected CD33m on Western blots. The antibody was also shown to work in immunocytochemical applications, staining CD33-transfected HEK cells (not shown).

Monoclonal antibody 10B2 recognizes human CD33m overexpressed in HEK cells. A) Western blots of lysates of HEK293 cells transfected with myc-tagged human CD33 isoforms or empty vector (mock). Antibody 10B2 preferentially detects CD33m. B) Antibody 10B2 also preferentially recognizes CD33m when used as a capture antibody for immunoprecipitation. Left blot, cell lysates immunoprecipitated with 10B2; right blot, cell lysates (input for immunoprecipitation). Blots probed with anti-myc antibody 9E10. *, non-specific bands. [Courtesy of Christian Haass' laboratory.]

Generation and epitope mapping

This antibody was generated in rats immunized with a peptide in the C-terminal region of human CD33 that is present in all three isoforms, coupled to ovalbumin. Epitope mapping has not been reported.

Specificity

Based on the amino acid sequence of the peptide immunogen, clone 10B2 is expected to react with all three isoforms of the human protein. The antibody is not expected to react with mouse CD33.

Validation

No data.

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References

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Further Reading

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