Antibody

Amyloid-β (6F/3D)

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Clonality: monoclonal
Host: Mouse
Isotype: IgG1κ
Source: DAKO
Catalog #: M0872
Formulation: Cell culture supernatant dialysed against 50 mmol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3
Reactivity:
Reacts in: Human
Availability: Confirmed available June 2015

Immunogen/Epitope

immunogen = synthetic peptide consisting of residues 8-17 Aβ with additional C-terminal cysteine coupled to keyhole limpet hemocyanin (KLH) via the cysteine residue

Specificity

Targets extracellular β-amyloid in human brain parenchyma and cerebral vessels.

Note

According to the spec sheet on the Dako product page, formalin-fixed, paraffin-embedded tissue sections must be treated with formic acid prior to the immunohistochemical staining.

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Further Reading

Papers

  1. Ryu JK, Cho T, Choi HB, Wang YT, McLarnon JG. Microglial VEGF receptor response is an integral chemotactic component in Alzheimer's disease pathology. J Neurosci. 2009 Jan 7;29(1):3-13. PubMed.
  2. Leinonen V, Alafuzoff I, Aalto S, Suotunen T, Savolainen S, Någren K, Tapiola T, Pirttilä T, Rinne J, Jääskeläinen JE, Soininen H, Rinne JO. Assessment of beta-amyloid in a frontal cortical brain biopsy specimen and by positron emission tomography with carbon 11-labeled Pittsburgh Compound B. Arch Neurol. 2008 Oct;65(10):1304-9. PubMed.
  3. Houlden H, Johnson J, Gardner-Thorpe C, Lashley T, Hernandez D, Worth P, Singleton AB, Hilton DA, Holton J, Revesz T, Davis MB, Giunti P, Wood NW. Mutations in TTBK2, encoding a kinase implicated in tau phosphorylation, segregate with spinocerebellar ataxia type 11. Nat Genet. 2007 Dec;39(12):1434-6. PubMed.
  4. Bacskai BJ, Frosch MP, Freeman SH, Raymond SB, Augustinack JC, Johnson KA, Irizarry MC, Klunk WE, Mathis CA, Dekosky ST, Greenberg SM, Hyman BT, Growdon JH. Molecular imaging with Pittsburgh Compound B confirmed at autopsy: a case report. Arch Neurol. 2007 Mar;64(3):431-4. PubMed.
  5. Mastrianni JA, Capellari S, Telling GC, Han D, Bosque P, Prusiner SB, Dearmond SJ. Inherited prion disease caused by the V210I mutation: transmission to transgenic mice. Neurology. 2001 Dec 26;57(12):2198-205. PubMed.
  6. Akiyama H, Mori H, Saido T, Kondo H, Ikeda K, McGeer PL. Occurrence of the diffuse amyloid beta-protein (Abeta) deposits with numerous Abeta-containing glial cells in the cerebral cortex of patients with Alzheimer's disease. Glia. 1999 Feb 15;25(4):324-31. PubMed.
  7. Akiyama H, Schwab C, Kondo H, Mori H, Kametani F, Ikeda K, McGeer PL. Granules in glial cells of patients with Alzheimer's disease are immunopositive for C-terminal sequences of beta-amyloid protein. Neurosci Lett. 1996 Mar 15;206(2-3):169-72. PubMed.
  8. Matsunaga Y, Saito N, Fujii A, Yokotani J, Takakura T, Nishimura T, Esaki H, Yamada T. A pH-dependent conformational transition of Abeta peptide and physicochemical properties of the conformers in the glial cell. Biochem J. 2002 Feb 1;361(Pt 3):547-56. PubMed.
  9. Papaioannou N, Tooten PC, van Ederen AM, Bohl JR, Rofina J, Tsangaris T, Gruys E. Immunohistochemical investigation of the brain of aged dogs. I. Detection of neurofibrillary tangles and of 4-hydroxynonenal protein, an oxidative damage product, in senile plaques. Amyloid. 2001 Mar;8(1):11-21. PubMed.