Updated 3 February 2011
Targeting of the Tardbp gene by the gene trapping vector pGT1lxf. Confirmed the
insertion of the pGT1lxf genetrap cassette within intron 2 of Tardbp at base pair
1226 of ES cell line RB030. The TardbpGt(RB030)Byg chimeric mice were
generated in the murine targeted genomics laboratory at the MMRRC from the RB030
Mouse strain: C57BL/6. Origin: C57BL/6/129. Backcross to both B6 and 129. The TardbpGt(RB030)Byg
heterozygous mice were backcrossed to C57BL/6 mice five times. Homozygous are embryonic
Het mice have no evidence of pathologic changes in motor neurons.
Homozygous TDP-43 are embryonic lethal. Het mice exhibit signs of motor disturbance
and muscle weakness with decreased forelimb grip strength and deficits in inverted
Contact: B.C. Kraemer
Geriatrics Research Education and Clinical Center, Veterans Affairs
Puget Sound Health Care System, Seattle, WA 98108, Phone: 206-277-1071
Kraemer BC, Schuck T, Wheeler JM, Robinson LC, Trojanowski JQ, Lee VM, Schellenberg
GD. Loss of murine TDP-43 disrupts motor function and plays an essential role in
embryogenesis. Acta Neuropathol. 2010 Apr;119(4):409-19.