Updated 25 January 2006
Transgene: The three FTDP-17 linked mutations G272V (V), P301L (L) and R406W (W)
were incorporated by site-directed mutagenesis of human CNS tau cDNA (2 N-terminal
inserts, 4 microtubule-binding repeats) then inserted into a murine Thy1 cassette
between exons 2 and 4.
Mutation: FTDP-17; G272V, P301L and R406W
Promoter: Mouse Thy-1 promoter with deleted lymphoid enhancer
Mouse Strain: C57Bl6j
Tauvlw mice overexpress human mutant Tau in cortex and hippocampus with
minimal expression in the spinal cord. Immunohistochemical analysis reveals high
transgene expression in neuronal cell bodies and neurites in the cortex and the
hippocampal formation. Ultrastructural analysis shows a pretangle appearance in
neurons expressing mutant tau, with filaments of tau and increased numbers of lysosomes
displaying aberrant morphology similar to those found in AD
Centro de Biología Molecular "Severo Ochoa," Facultad de Ciencias
Universidad Autónoma de Madrid
Lim, F., Hernández F., Lucas J.J., Gómez-Ramos P., Morán M.A. and Ávila J. FTDP-17
mutations in tau transgenic mice provoke lysosomal abnormalities and Tau filaments
in forebrain. Mol. Cell. Neurosci. 18: 702-4, 2001.
Ferrer I, Barrachina M, Puig B, Martinez de Lagran M, Marti E, Avila J, Dierssen
M. Constitutive Dyrk1A is abnormally expressed in Alzheimer disease, Down syndrome,
Pick disease, and related transgenic models. Neurobiol Dis. 2005 Nov;20(2):392-400.
Perez M, Hernandez F, Lim F, Diaz-Nido J, Avila J. Chronic lithium treatment decreases
mutant tau protein aggregation in a transgenic mouse model. J Alzheimers Dis. 2003