Posted 7 October 2002
Transgene: Longest human tau isoform (htau40) cDNA
Promoter: murine prion protein promoter with tetracycline-dependent transactivator
Mouse Strain: B6DF21
Human tau was expressed at high levels in neurons and oligodendrocytes. Tau-positive
neurons were found primarily in brainstem, deep cerebellar nuclei, and spinal cord.
Tau-positive oligodendrocytes were found throughout brain and spinal cord.
Human tau-specific antibodies (HT7 or Tau14) showed "extensive, clod-like, pretangle
accumulations" of tau in neuronal and oligodentrocytic soma and processes.
Tau filiments were found in oligodendrocytes had either straight or twisted structures;
17-20 nm wide with periodicity of ~75nm.
AT8 staining identified tau phosphorylation at residues 202/205 in oligodendrocytes
but not in neurons.
Thioflavin-S staining showed fibrillary inclusions in both oligodendrocytes and
neurons in spinal cord, but only in oligodendrocytes in brainstem.
No neurological deficits readily noticeable.
Gotz J, Tolnay M, Barmettler R, Chen F, Probst A, Nitsch RM. Oligodendroglial tau
filament formation in transgenic mice expressing G272V tau. Eur J Neurosci. 2001;13:2131-40.