Transgene: PS2 cDNA reverse transcribed from normal aged human brain
Mutation: N141I missense mutation
Promoter: chicken b-actin promoter
Coding region: EcoRI-BglII fragment subcloned into pCAGGS, joined to the cytomegalovirus
immediate early enhancer sequence, the chicken b-actin promoter, and the rabbit
b-globin polyadenylation signal.
Mouse Strain: C57BL/6 (B6)J or DBF1 fremales and B6N males
Steady-state level of human wild-type and mutant PS2 mRNA was five (mutant)
to fifteen (wildtype) higher than that of the endogenous mouse PS2 mRNA.
Ab42 levels appeared to be increase steadily in the mutant PS2 transgenic mouse
brains from 2 to 8 months of age, whereas there was only a small increase in wild-type
transgenic mice between the age of 5 and 8 months. Similar age-dependent increase
in the Ab42 levels as seen in the mutant Tg is also observed in nontransgenic mice.
The insoluble Ab levels were found to be higher than the soluble Ab levels, and
the insoluble Ab42 levels were markedly increased in mutant PS2 transgenic mice.
Not commercially available.
Contact Dr. Yasuo Ihara
Department of Neuropathology
University of Tokyo
Bunkyo-ku, Tokyo 113-0033
Oyama F, Sawamura N, Kobayashi K, Morishima-Kawashima M, Kuramochi T, Ito M, Tomita
T, Maruyama K, Saido TC, Iwatsubo T, Capell A, Walter J, Grunberg J, Ueyama Y, Haass
C, Ihara Y. Mutant presenilin 2 transgenic mouse: effect on an age-dependent increase
of amyloid beta-protein 42 in the brain. J Neurochem 1998 Jul;71(1):313-22.
Sawamura N, Morishima-Kawashima M, Waki H, Kobayashi K, Kuramochi T, Frosch MP,
Ding K, Ito M, Kim TW, Tanzi RE, Oyama F, Tabira T, Ando S, Ihara Y. Mutant presenilin
2 transgenic mice. A large increase in the levels of Abeta 42 is presumably associated
with the low density membrane domain that contains decreased levels of blycerophospholipids
and sphingomyelin. J Biol Chem 2000 Sep 8;275(36):27901-8.