Updated 23 January 2009
Transgene: Neuron-specific conditional KO of PS1, denoted PS1 (n-/-) using Cre/lox
technique. A "floxed" PS1 allele was constructed to contain three loxP sites and
a neomycin resistance cassette (neoR) by gene targeting in embryonic stem cells.
Two loxP sites flanked exon 7 of the mouse PS1 gene and the third loxP site was
placed downstream of the neoR mrker. Thy-1 Cre-recombinase transgenic mice were
generated. Neuron specific Thy-1 driven Cre-recombinase was assessed by crossing
with LacZ reporter mice. The PS1(n-/-) mice are bi-genic mice, generated by crossing:
(1) transgenic mice with a "floxed" PS1 gene containing 3 loxP sites and (2) mice
with neuron-specific expression of Cre-recombinase under control of the mouse thy1-gene
Mutation: Deletion of exon 7 of the PS1 gene
Promoter: Mouse Thy1 gene promoter (driving Cre-recombinase to neurons)
Mouse Strain: B6;129P-Psen1tm1Vln
In adult PS1 (n-/-) mice, levels of murine brain Aß40 and 42 were strongly decreased,
concomitant with accumulation of amyloid precursor protein (APP) C-terminal fragments.
PS1(n-/-) are viable and fertile with normal brain morphology.
Mice can be made available for academic collaboration under MTA with KULeuven-R&D
The Jackson Lab,
available, stock #007605. Use by companies or for-profit entities requires a license
prior to shipping.
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M, Moechars D, Laenen I, Kuiperi C, Bruynseels K, Tesseur I, Loos R, Vanderstichele
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