Posted 9 June 2005
Transgene: Double transgenic PS2APP mice were bred by crossing homozygous APPswe
(line 71) males with PS2mut (line 30) females
Mutation: APP (K670N, M671L) and PS2 (N141I)
Promoter: Murine prion protein genomic fragment (pPrnpHG) was used for PS2mThy-1.2
At 8 months, PS2APP mice develop age-related cognitive deficits and correlative
amyloid deposits with inflammation in neo- and limbic cortices, including the hippocampal
formation and amygdala, as well as thalamic and pontine nuclei. The metabolic profile
showed clear indication of hypometabolism with age (20m) in the PS2APP mice: both
N-acetyl-aspartate and glutamate were significantly reduced in the older animals.
These spectroscopic measures in vivo correlated well with the plaque load in the
Behavioral changes begin when Aß deposits and inflammation appear in the subiculum
and frontolateral cortex.
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J.G. Richards, G.A. Higgins, A.M. Ouagazzal, L. Ozmen, J.N. Kew, B. Bohrmann, P.
Malherbe, M. Brockhaus, H. Loetscher, C. Czech, G. Huber, H. Bluethmann, H. Jacobsen
and J.A. Kemp. PS2APP transgenic mice, coexpressing hPS2mut and hAPPswe, show age-related
cognitive deficits associated with discrete brain amyloid deposition and inflammation,
J. Neurosci. 23:8989-9003, 2003.
von Kienlin M, Künnecke B, Metzger F, Steiner G, J. Richards G, Ozmen L, Jacobsen
H, Loetscher H. Altered metabolic profile in the frontal cortex of PS2APP transgenic
mice, monitored throughout their life span. Neurobiology of Dis. 18(1):32-39, 2005.