Updated 14 April 2005
Transgene: Human wild-type αSYN was first subcloned into the XhoI site of a
modified intermediate vector derived from pNEB193. This construct was digested with
AscI and PacI, and the insert was subcloned into an OL-specific proteolipid protein
promoter (PLP) cassette that was modified to contain a multiple cloning site after
the 3' boundary of intron 1. A linear ApaI-SacII fragment of 11 kb comprising the
transgene without plasmid sequences was isolated and injected into C57Bl/6 DBA/2
Promoter: Proteolipid protein promotor
Mouse Strain: C57BL/6. Backcrossed to generation N5
Transgenic αSYN was detected in brain white matter and oligodendrocytes bodies
but no other brain cell type. It was detergent insoluble. Hyperphosphorylation at
S129 of αSyn in the tg mice.
Lab of Alzheimer's and Parkinson's Disease Res, Dept Biochemisty
Ludwig Maximilians University of Munich
Schillerstrasse 44, 80336, Munich, Germany
(+49-89) 5996-480, 475
Kahle PJ, Neumann M, Ozmen L, Muller V, Jacobsen H, Spooren W, Fuss B, Mallon B,
Macklin WB, Fujiwara H, Hasegawa M, Iwatsubo T, Kretzschmar HA, Haass C. Hyperphosphorylation
and insolubility of alpha-synuclein in transgenic mouse oligodendrocytes. EMBO Rep.
2002 Jun 1 ; 3(6):583-8.
Stefanova N, Reindl M, Neumann M, Haass C, Poewe W, Kahle PJ, Wenning GK. Oxidative
stress in transgenic mice with oligodendroglial alpha-synuclein overexpression replicates
the characteristic neuropathology of multiple system atrophy. Am J Pathol. 2005