Posted 13 June 2005
Transgene: The construct contained the human BACE cDNA inserted in the mouse thy-1
gene cassette at the XhoI site of the pTSC plasmid. Final constructs were linearized,
purified and microinjected into pronuclear DBA/C57BL6 embryos.
Mutation: huBACE (wt)
Promoter: Mouse Thy1
Mouse Strain: The five resulting BACE transgenic founders were expanded into the
Transgene expressed in neurons only. Line 16 had high expression of human BACE.
Department of Biochemistry
Laboratory for Alzheimer's and Parkinson's Disease Research
Schillerstr. 44, 80336 Munich
Phone: 089 - 2180 75 - 471
Fax: 089 - 2180 75 - 415
Note: Embryos are stored at Charles River Breeding Labs.
Willem M, Dewachter I, Smyth N, Van Dooren T, Borghgraef P, Haass C, Van Leuven
F. b-site amyloid precursor protein cleaving enzyme 1 increases amyloid deposition
in brain parenchyma but reduces cerebrovascular amyloid angiopathy in aging BACE
x APP(V717I) double-transgenic mice. Am J Path. 105(5): 1621-1631, 2004.