Posted 21 April 2005
Transgene: The Arctic mutation was introduced into a hAPP minigene (having Swedish
and Indiana mutations) directed by the platelet-derived growth factor β-chain
promoter. Two hAPP-Arc lines (Arc6 and Arc48) were established.
Mutation: Arctic (E22G), Swedish, and Indiana
Promoter: Platelet-derived growth factor βchain promoter
Compared to J20 mice (TJL #004661) the Arctic mice formed amyloid plaques faster
and more extensively, even though they had lower Aβ 1-42/1-40 ratios. Relative
levels of hAPP and of the β-secretase-generated C99 fragment were lower in
Arc6 and higher in Arc48 than in J20 mice.
Lennart Mucke, MD
Gladstone Institute of Neurological Disease
1650 Owens Street
San Francisco, CA 94158
Cheng I H, Palop J J, Esposito L A, Bien-Ly N, Yan F, Mucke L. Aggressive amyloidosis
in mice expressing human amyloid peptides with the Arctic mutation. Nat Med. 10(11):
1190-2, 2004. Abstract.
Mucke L, Masliah E, Yu G-O, Mallory M, Rockenstein E M, Tatsuno G, Hu K, Kholodenko
D, Johnson-Wood K, McConlogue L. High-level neuronal expression of aβ1-42 in
wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without
plaque formation. J Neurosci 20:4050-4058, 2000.