Posted 14 November 2008
Transgene: Human APP751 cDNA with the E693Q mutation was inserted into the blunt-ended
XhoI site of the vector pTSC 1 containing the murine Thy1.2 minigene. After removal
of vector sequences by NotI/PvuI digestion, linear Thy1-APPconstructs were injected
into C57BL/6J oocytes.
Mutation: E693Q (Dutch)
Promoter: Mouse Thy1.2
Strain: C57BL/6J-Tg(Thy1-APPDutch); original background strain: C57BL/6J
APP Dutch mice develop cerebral amyloid angiopathy (in the absence of parenchymal
amyloid) at approx. 24 mo of age, Aβ40 >> Aβ42. The line with the highest
transgene expression is approx. 5x greater than endogenous APP levels.
Contact Mathias Jucker, firstname.lastname@example.org.
Herzig MC, Winkler DT, Burgermeister P, Pfeifer M, Kohler E, Schmidt SD, Danner
S, Abramowski D, Stürchler-Pierrat C, Bürki K, van Duinen SG, Maat-Schieman MLC,
Staufenbiel M, Mathews PM, Jucker M. Abeta is targeted to the vasculature in a mouse
model of hereditary cerebral hemorrhage with amyloidosis. Nature Neuroscience 2004;