Transgene: Mouse APP genomic DNA, contained within universal targeting vector pPNTlox
Mutation: Site-specific mutation of three codons in exon 16 of mouse (129/SV) APP
sequence to (1) introduce the two Swedish familial AD mutations; and (2) "humanize"
murine Aβ sequence by altering three amino acids
Mouse Strain: Mice generated from R1 line of embryonic stem cells
In brain, mutant mouse APP with humanized Aβ domain is expressed at levels
comparable to mouse APP in wild type.
Amyloidogenic processing of the mutant mouse APP at the β-secretase site is
"Human" Aβ found in mutant mice at levels nine-fold greater than that found
in normal human brain.
Contact Dorothy Flood at email@example.com
or Steve Trusko firstname.lastname@example.org
Reaume AG, Howland DS, Trusko SP, Savage MJ, Lang DM, Greenberg BD, Siman R, Scott
RW. Enhanced amyloidogenic processing of the β-amyloid precursor protien in
gene-targeted mic bearing the Swedish familial Alzheimer's disease mutations and
"humanized" Aβ sequence. J Biol Chem. 20 Sept 1996;271(38):23380-8.