Brief Description:
3x Bromophenol Blue Sample Buffer.
Recipe:
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3x BPB with 5% BME
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1x
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3x
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Stock solutions
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Tris HCl
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63 mM
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189
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2M pH 6.8
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Glycerol
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10%
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30%
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100%
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SDS
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2%
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5%
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20%
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BPB
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0.0025%
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0.0075
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0.5%
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H20
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β-ME
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5%
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15%
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100%
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More concentrated sample buffer can be made by reducing the amount of SDS and β- Mercaptoethanol.