Updated 7 September 2010
Brief Description:
This is our protocol to prepare primary cultures consisting of >95% microglia from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Our protocols for preparing
mixed glial or
highly enriched astroglial cultures are also available at the Alzforum database.
Protocol Procedure: View Protocol
References:
Saura J, Tusell JM, Serratosa J. High-yield isolation of murine microglia by mild trypsinization. Glia. 2003 Dec;44(3):183-9.
Abstract