Updated 7 September 2010
Protocol Procedure: View Protocol
This is our protocol to prepare primary cultures consisting of >95% microglia from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Our protocols for preparing mixed glial
or highly enriched astroglial cultures
are also available at the Alzforum database.
Saura J, Tusell JM, Serratosa J. High-yield isolation of murine microglia by mild trypsinization. Glia. 2003 Dec;44(3):183-9. Abstract