Updated 7 September 2010

This is our protocol to prepare primary glial cultures consisting of 75% astrocytes and 25% microglia approximately. We use neonatal mouse cerebral cortex, but the protocol also works for late embryos, for rat, and for most CNS regions. Our protocols for preparing highly enriched astroglial or microglial cultures are also available at the Alzforum database.

Saura J. Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation. 2007 Oct 15;4:26. Review. Abstract