Updated 6 June 2010
| Protocol: | Efficient Transfection of Murine Embryonic Motor Neurons via Magnetofection |
| |
| Submitted By: | Wilfried Rossoll |
| Lab: |
Bassell Lab |
| PI Name: |
Gary J. Bassell |
Brief Description:
An optimized protocol for the efficient transfection of cultured primary motor neurons via magnetofection, a novel transfection technology based on the delivery of DNA-coated magnetic nanobeads.
Protocol Procedure: View Protocol (Download .PDF)
Equipment:
MF10000 Super Magnetic Plate or MF14000 Mega Magnetic Plate (Oz Biosciences).
NM51000 NeuroMag transfection reagent (Oz Biosciences)
References:
Fallini C, Bassell GJ, Rossoll W. High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking and function. Mol Neurodegener. 2010 Apr 21;5:17.
Abstract