Updated 19 June 2009
| Protocol: | In vivo Cathepsin D and Cathepsin B Activity Assay |
| |
| Submitted By: | Ralph Nixon |
| Lab: |
Nixon |
| PI Name: |
Ralph A. Nixon |
Brief Description:
In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in live cell condition.
Protocol Procedure: View Protocol
Equipment:
plan-Apochromat 40x or 100x/1.4 oil DIC objective lens on the laser scanning confocal Microscope, LSM 510 META, with LSM software v3.5 (Carl Zeiss MicroImaging Inc, Thornwood, NY, USA).
Images were analyses using ImageJ program (NIH).
References:
Chen, C.-S., Chen, W.-N.U., Zhou, M., Arttamangkul, S., and Haugland, R.P. (2000). Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. Journal of Biochemical and Biophysical Methods 42, 137-151.
Abstract
Erwig, L.-P., Mcphilips, K.A., Wynes, M.W., Ivetic, A., Ridley, A.J., And Henson, P.M. (2006). Differential Regulation Of Phagosome Maturation In Macrophages And Dendritic Cells Mediated By Rho Gtpases And Ezrin-Radixin-Moesin (Erm) Proteins. Proceedings Of The National Academy Of Sciences 103, 12825-12830. abstract
Yang DS, Lee JH, Nixon RA. Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. Abstract