PI: Ralph Nixon
Affiliation: New York University School of Medicine and the Nathan Kline Institute
Submitted by: Ralph Nixon
Posted Date: 19 Jun 2009

Brief Description

In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in living cells.

References

1. Chen CS, Chen WN, Zhou M, Arttamangkul S, Haugland RP. Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. J Biochem Biophys Methods. 2000 Mar 16;42(3):137-51. PubMed.
2. Erwig LP, McPhilips KA, Wynes MW, Ivetic A, Ridley AJ, Henson PM. Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12825-30. Epub 2006 Aug 14 PubMed.
3. Yang DS, Lee JH, Nixon RA. Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. PubMed.

Keywords

lysosome, Cat D, Cat B, confocal microscopy

Further Reading

No Available Further Reading