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In vivo Cathepsin D and Cathepsin B Activity Assay

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PI: Ralph Nixon
Affiliation: New York University School of Medicine and the Nathan Kline Institute
Submitted by: Ralph Nixon
Posted Date: 19 Jun 2009

Brief Description

In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in living cells.

References

  1. . Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. J Biochem Biophys Methods. 2000 Mar 16;42(3):137-51. PubMed.
  2. . Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12825-30. Epub 2006 Aug 14 PubMed.
  3. . Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. PubMed.

Keywords

lysosome, Cat D, Cat B, confocal microscopy

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