Protocols

Primary Support Cultures of Hippocampal and Substantia Nigra Neurons

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PI: Jürgen Götz
Affiliation: University of Sydney
Submitted by: Jürgen Götz
Posted Date: 13 Apr 2009

Brief Description

This protocol describes how to culture embryonic (E16.5) murine hippocampal neurons using a spatially separated ring of cortical neurons for neurotrophic support. This method allows long-term cultures at a very low cell density, and therefore, the study of single embryo preparations and isolated neurons. This method has been adopted for neurons from the substantia nigra (E16.5) with support from a ring of striatal neurons. This protocol is available through Nature Protocols.

References

  1. . Primary support cultures of hippocampal and substantia nigra neurons. Nat Protoc. 2009;4(1):78-85. PubMed.
  2. . Parkinsonism and impaired axonal transport in a mouse model of frontotemporal dementia. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15997-6002. Epub 2008 Oct 2 PubMed.

Keywords

neuron, primary cell, hippocampal, hippocampus, mouse

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